3kg4

Structural highlights

Function

CAS5D_MANSM CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein is a sequence-specific endonuclease that cleaves pre-crRNA at G21 into mature crRNA. Does not cleave pre-crRNA associated with the T.thermophilus strain HB27 Cas5 protein (AC Q746C2) CRISPR locus (PubMed:23006625). The reaction mechanism may proceed by an intramolecular attack of the 2'-hydroxyl group of G21 on the scissile phosphodiester, cutting the precursor 3' to G21 residue yielding 5'-hydroxyl and 2' and/or 3' ends lacking a hydroxyl group (perhaps a 2'/3' cyclic phosphodiester) (Ref.2).^{[1] [2]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• CRISPR subtype I-C
• CRISPR-Cas

References

1. ↑ Garside EL, Schellenberg MJ, Gesner EM, Bonanno JB, Sauder JM, Burley SK, Almo SC, Mehta G, MacMillan AM. Cas5d processes pre-crRNA and is a member of a larger family of CRISPR RNA endonucleases. RNA. 2012 Nov;18(11):2020-8. doi: 10.1261/rna.033100.112. Epub 2012 Sep 24. PMID:23006625 doi:http://dx.doi.org/10.1261/rna.033100.112
2. ↑ Garside EL, Schellenberg MJ, Gesner EM, Bonanno JB, Sauder JM, Burley SK, Almo SC, Mehta G, MacMillan AM. Cas5d processes pre-crRNA and is a member of a larger family of CRISPR RNA endonucleases. RNA. 2012 Nov;18(11):2020-8. doi: 10.1261/rna.033100.112. Epub 2012 Sep 24. PMID:23006625 doi:http://dx.doi.org/10.1261/rna.033100.112