3kw8

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Two-domain laccase from Streptomyces coelicolor at 2.3 A resolution

Structural highlights

3kw8 is a 1 chain structure with sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.29Å
Ligands:CU, FE, NA, PG4, PGE
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9XAL8_STRCO

Publication Abstract from PubMed

The paper reports the structure of the small laccase from Streptomyces coelicolor determined from a crystal soaked with potassium hexacyanoferrate [K4Fe(CN)6]. The decolorization of the natively blue crystal observed upon soaking indicates the reduction of the enzyme in the crystal. The ligand binds between laccase molecules and stabilizes the crystal. The increased diffraction limit of the diffraction data collected from this crystal enabled the refinement of the small laccase structure at 2.3 A resolution, which is the highest resolution obtained to date.

Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 A.,Skalova T, Duskova J, Hasek J, Stepankova A, Koval T, Ostergaard LH, Dohnalek J Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt, 1):27-32. Epub 2010 Dec 21. PMID:21206017[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. Skalova T, Duskova J, Hasek J, Stepankova A, Koval T, Ostergaard LH, Dohnalek J. Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 A. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt, 1):27-32. Epub 2010 Dec 21. PMID:21206017 doi:10.1107/S1744309110046099

Contents


PDB ID 3kw8

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