3mm0

Publication Abstract from PubMed

Avidin and its bacterial analogue streptavidin have been widely used in applications in life sciences. Recently, we described a highly thermostable engineered avidin, called chimeric avidin, which is a hybrid of avidin and avidin related protein 4. Here we report a protocol for pilot-scale production in E. coli and the X-ray structure of the Ile117Tyr mutant of chimeric avidin. The ligand-binding properties of chimeric avidin were explored with isothermal titration calorimetry. We found chimeric avidin to be more stable against various harsh organic solvents at elevated temperatures compared to avidin and streptavidin. The properties of chimeric avidin make it a potential tool for new applications in biotechnology. (c) 2010 Wiley Periodicals, Inc.

Chimeric avidin shows stability against harsh chemical conditions - biochemical analysis and 3D structure.,Maatta JA, Eisenberg-Domovich Y, Nordlund HR, Hayouka R, Kulomaa MS, Livnah O, Hytonen VP Biotechnol Bioeng. 2010 Oct 11. PMID:20939005^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.