3mzq

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RNase crystals grown by the hanging drop method

Structural highlights

3mzq is a 1 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Ligands:CL, SO4
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RNAS1_BOVIN Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.[1]

Publication Abstract from PubMed

Protein crystals are usually grown in hanging or sitting drops and generally get transferred to a loop or micromount for cryocooling and data collection. This paper describes a method for growing crystals on cryoloops for easier manipulation of the crystals for data collection. This study also investigates the steps for the automation of this process and describes the design of a new tray for the method. The diffraction patterns and the structures of three proteins grown by both the new method and the conventional hanging-drop method are compared. The new setup is optimized for the automation of the crystal mounting process. Researchers could prepare nanolitre drops under ordinary laboratory conditions by growing the crystals directly in loops or micromounts. As has been pointed out before, higher levels of supersaturation can be obtained in very small volumes, and the new method may help in the exploration of additional crystallization conditions.

Diffraction study of protein crystals grown in cryoloops and micromounts.,Berger MA, Decker JH, Mathews II J Appl Crystallogr. 2010 Dec 1;43(Pt 6):1513-1518. Epub 2010 Oct 20. PMID:22477781[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
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Direct cryocooling of naked crystals: are cryoprotection agents always necessary?
Pellegrini et al. (2011)
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See Also

References

  1. ↑ delCardayre SB, Ribo M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT. Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11. Protein Eng. 1995 Mar;8(3):261-73. PMID:7479688
  2. ↑ Berger MA, Decker JH, Mathews II. Diffraction study of protein crystals grown in cryoloops and micromounts. J Appl Crystallogr. 2010 Dec 1;43(Pt 6):1513-1518. Epub 2010 Oct 20. PMID:22477781 doi:10.1107/S0021889810040409

Contents


PDB ID 3mzq

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