4b5c

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Crystal structure of the peptidoglycan-associated lipoprotein from Burkholderia pseudomallei

Structural highlights

4b5c is a 3 chain structure with sequence from Burkholderia pseudomallei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:ACT
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q63RA7_BURPS

Publication Abstract from PubMed

We solved the crystal structure of Burkholderia pseudomallei acute phase antigen BPSL2765 in the context of a structural vaccinology study, in the area of melioidosis vaccine development. Based on the structure, we applied a recently developed method for epitope design that combines computational epitope predictions with in vitro mapping experiments and successfully identified a consensus sequence within the antigen that, when engineered as a synthetic peptide, was selectively immunorecognized to the same extent as the recombinant protein in sera from melioidosis-affected subjects. Antibodies raised against the consensus peptide were successfully tested in opsonization bacterial killing experiments and antibody-dependent agglutination tests of B. pseudomallei. Our strategy represents a step in the development of immunodiagnostics, in the production of specific antibodies and in the optimization of antigens for vaccine development, starting from structural and physicochemical principles.

Exploiting the Burkholderia pseudomallei acute phase antigen BPSL2765 for structure-based epitope discovery/design in structural vaccinology.,Gourlay LJ, Peri C, Ferrer-Navarro M, Conchillo-Sole O, Gori A, Rinchai D, Thomas RJ, Champion OL, Michell SL, Kewcharoenwong C, Nithichanon A, Lassaux P, Perletti L, Longhi R, Lertmemongkolchai G, Titball RW, Daura X, Colombo G, Bolognesi M Chem Biol. 2013 Sep 19;20(9):1147-56. doi: 10.1016/j.chembiol.2013.07.010. Epub, 2013 Aug 29. PMID:23993463[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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References

  1. Gourlay LJ, Peri C, Ferrer-Navarro M, Conchillo-Sole O, Gori A, Rinchai D, Thomas RJ, Champion OL, Michell SL, Kewcharoenwong C, Nithichanon A, Lassaux P, Perletti L, Longhi R, Lertmemongkolchai G, Titball RW, Daura X, Colombo G, Bolognesi M. Exploiting the Burkholderia pseudomallei acute phase antigen BPSL2765 for structure-based epitope discovery/design in structural vaccinology. Chem Biol. 2013 Sep 19;20(9):1147-56. doi: 10.1016/j.chembiol.2013.07.010. Epub, 2013 Aug 29. PMID:23993463 doi:http://dx.doi.org/10.1016/j.chembiol.2013.07.010

Contents


PDB ID 4b5c

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