4iug

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Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose

Structural highlights

4iug is a 1 chain structure with sequence from Aspergillus oryzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Ligands:BMA, CD, GAL, M6D, MAN, NAG, SEP
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BGALA_ASPOR Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans (By similarity).

Publication Abstract from PubMed

The crystal structure of the industrially important Aspergillus oryzae beta-galactosidase has been determined at 2.60A resolution. The Ao-beta-gal is a large (985 residues) monomeric multi-domain enzyme that has a catalytic (alpha/beta)8-barrel domain. An electron density map revealed extensive N-glycosylation between the domain interfaces suggesting that the oligosaccharide-chains would have a stabilizing role for the structure of Ao-beta-gal. Comparison of structure with other beta-galactosidase structures of glycoside hydrolase family 35 revealed a number of hydrophobic residues, which may contribute favorably to the stabilization of the structure. The role of a high number of acidic residues in Ao-beta-gal is also discussed.

The crystal structure of acidic beta-galactosidase from Aspergillus oryzae.,Maksimainen MM, Lampio A, Mertanen M, Turunen O, Rouvinen J Int J Biol Macromol. 2013 Sep;60:109-15. doi: 10.1016/j.ijbiomac.2013.05.003., Epub 2013 May 17. PMID:23688418[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
2 reviews cite this structure
Cold-Active β-Galactosidases: Insight into Cold Adaption Mechanisms and Biotechnological Exploitation.
Mangiagalli et al. (2021)
1 more
23 other articles
No citations found

See Also

References

  1. ↑ Maksimainen MM, Lampio A, Mertanen M, Turunen O, Rouvinen J. The crystal structure of acidic beta-galactosidase from Aspergillus oryzae. Int J Biol Macromol. 2013 Sep;60:109-15. doi: 10.1016/j.ijbiomac.2013.05.003., Epub 2013 May 17. PMID:23688418 doi:10.1016/j.ijbiomac.2013.05.003

Contents


PDB ID 4iug

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OCA

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