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4kfv

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Structural insight into Golgi membrane stacking by GRASP65 and GRASP55

Structural highlights

4kfv is a 1 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GORS1_RAT Key structural protein of the Golgi apparatus (By similarity). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (By similarity). Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (By similarity). However, other studies suggest that GORASP1 plays an important role in assembly and membrane stacking of the cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis (PubMed:12839990, PubMed:23940043, PubMed:9346242). Caspase-mediated cleavage of GORASP1 is required for fragmentation of the Golgi during apoptosis (PubMed:11815631, PubMed:12015985). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity).[UniProtKB:Q91X51][UniProtKB:Q9BQQ3]^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Lane JD, Lucocq J, Pryde J, Barr FA, Woodman PG, Allan VJ, Lowe M. Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis. J Cell Biol. 2002 Feb 4;156(3):495-509. Epub 2002 Jan 28. PMID:11815631 doi:http://dx.doi.org/10.1083/jcb.200110007
↑ Sutterlin C, Hsu P, Mallabiabarrena A, Malhotra V. Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells. Cell. 2002 May 3;109(3):359-69. doi: 10.1016/s0092-8674(02)00720-1. PMID:12015985 doi:http://dx.doi.org/10.1016/s0092-8674(02)00720-1
↑ Wang Y, Seemann J, Pypaert M, Shorter J, Warren G. A direct role for GRASP65 as a mitotically regulated Golgi stacking factor. EMBO J. 2003 Jul 1;22(13):3279-90. PMID:12839990 doi:http://dx.doi.org/10.1093/emboj/cdg317
↑ Feng Y, Yu W, Li X, Lin S, Zhou Y, Hu J, Liu X. Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins. J Biol Chem. 2013 Sep 27;288(39):28418-27. doi: 10.1074/jbc.M113.478024. Epub, 2013 Aug 12. PMID:23940043 doi:http://dx.doi.org/10.1074/jbc.M113.478024
↑ Barr FA, Puype M, Vandekerckhove J, Warren G. GRASP65, a protein involved in the stacking of Golgi cisternae. Cell. 1997 Oct 17;91(2):253-62. PMID:9346242