4m2z

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Crystal structure of RNASE III complexed with double-stranded RNA and CMP (TYPE II CLEAVAGE)

Structural highlights

4m2z is a 4 chain structure with sequence from Aquifex aeolicus VF5 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.85Å
Ligands:C5P, MG
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RNC_AQUAE Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon.[1] [2]

Publication Abstract from PubMed

RNase III is a global regulator of gene expression in Escherichia coli that is instrumental in the maturation of ribosomal and other structural RNAs. We examine here how RNase III itself is regulated in response to growth and other environmental changes encountered by the cell and how, by binding or processing double-stranded RNA (dsRNA) intermediates, RNase III controls the expression of genes. Recent insight into the mechanism of dsRNA binding and processing, gained from structural studies of RNase III, is reviewed. Structural studies also reveal new cleavage sites in the enzyme that can generate longer 3' overhangs.

RNase III: Genetics and Function; Structure and Mechanism.,Court DL, Gan J, Liang YH, Shaw GX, Tropea JE, Costantino N, Waugh DS, Ji X Annu Rev Genet. 2013 Nov 23;47:405-31. doi: 10.1146/annurev-genet-110711-155618. PMID:24274754[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
19 reviews cite this structure
Comparative Structure and Function Analysis of the RIG-I-Like Receptors: RIG-I and MDA5.
Brisse et al. (2019)
18 more
68 other articles
No citations found

See Also

References

  1. ↑ Shi Z, Nicholson RH, Jaggi R, Nicholson AW. Characterization of Aquifex aeolicus ribonuclease III and the reactivity epitopes of its pre-ribosomal RNA substrates. Nucleic Acids Res. 2011 Apr;39(7):2756-68. doi: 10.1093/nar/gkq1030. Epub 2010, Dec 7. PMID:21138964 doi:http://dx.doi.org/10.1093/nar/gkq1030
  2. ↑ Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X. Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III. Cell. 2006 Jan 27;124(2):355-66. PMID:16439209 doi:10.1016/j.cell.2005.11.034
  3. ↑ Court DL, Gan J, Liang YH, Shaw GX, Tropea JE, Costantino N, Waugh DS, Ji X. RNase III: Genetics and Function; Structure and Mechanism. Annu Rev Genet. 2013 Nov 23;47:405-31. doi: 10.1146/annurev-genet-110711-155618. PMID:24274754 doi:http://dx.doi.org/10.1146/annurev-genet-110711-155618

Contents


PDB ID 4m2z

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OCA

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