4o6h

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2.8A crystal structure of Lymphocytic Choriomeningitis Virus Nucleoprotein C-terminal Domain

Structural highlights

4o6h is a 8 chain structure with sequence from Lymphocytic choriomeningitis virus (strain Armstrong). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Ligands:IMD, K, MG, ZN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NCAP_LYCVA Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3 phosphorylation and activation by host IKBKE. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of IRF3, a protein involved in IFN activation pathway, leading to the inhibition of IFNB and IRF3-dependent promoters activation.[1] [2] [3]

Publication Abstract from PubMed

The X-ray crystal structure of the Lymphocytic choriomeningitis virus nucleoprotein C-terminal immunosuppressive domain (LCMV NPDelta340) was determined to 2.0 A resolution. The structure indicates that LCMV NPDelta340, like the other structurally characterized arenaviral nucleoproteins, adopts the fold of an exonuclease. This structure provides a crucial three-dimensional template for functional exploration of the replication and immunosuppression of this prototypic arenavirus.

Structure of the LCMV nucleoprotein provides a template for understanding arenavirus replication and immunosuppression.,West BR, Hastie KM, Saphire EO Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1764-9. doi:, 10.1107/S1399004714007883. Epub 2014 May 30. PMID:24914986[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. ↑ Pinschewer DD, Perez M, de la Torre JC. Role of the virus nucleoprotein in the regulation of lymphocytic choriomeningitis virus transcription and RNA replication. J Virol. 2003 Mar;77(6):3882-7. PMID:12610166
  2. ↑ Martinez-Sobrido L, Giannakas P, Cubitt B, Garcia-Sastre A, de la Torre JC. Differential inhibition of type I interferon induction by arenavirus nucleoproteins. J Virol. 2007 Nov;81(22):12696-703. Epub 2007 Sep 5. PMID:17804508 doi:http://dx.doi.org/10.1128/JVI.00882-07
  3. ↑ Pythoud C, Rodrigo WW, Pasqual G, Rothenberger S, Martinez-Sobrido L, de la Torre JC, Kunz S. Arenavirus nucleoprotein targets interferon regulatory factor-activating kinase IKKepsilon. J Virol. 2012 Aug;86(15):7728-38. doi: 10.1128/JVI.00187-12. Epub 2012 Apr 24. PMID:22532683 doi:http://dx.doi.org/10.1128/JVI.00187-12
  4. ↑ West BR, Hastie KM, Saphire EO. Structure of the LCMV nucleoprotein provides a template for understanding arenavirus replication and immunosuppression. Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1764-9. doi:, 10.1107/S1399004714007883. Epub 2014 May 30. PMID:24914986 doi:http://dx.doi.org/10.1107/S1399004714007883

Contents


PDB ID 4o6h

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