| Structural highlights
Function
NMO_PSEAE Nitronate monooxygenase that uses molecular oxygen to catalyze the oxidative denitrification of alkyl nitronates. The toxin propionate 3-nitronate (P3N) is the best substrate (and the presumed physiological substrate), but this enzyme is also active on other primary and secondary nitronates such as propyl-1-nitronate, ethylnitronate, pentyl-1-nitronate, butyl-1-nitronate and propyl-2-nitronate (PubMed:25002579). Is likely involved in the degradation of P3N, that allows P.aeruginosa PAO1 to grow on 3-nitropropionate/P3N as the sole nitrogen source (PubMed:20382807). Also functions in the detoxification of P3N, a metabolic poison produced by plants and fungi as a defense mechanism (PubMed:25384477). Cannot oxidize nitroalkanes such as 3-nitropropionate, nitroethane, 1-nitropropane, 1-nitrobutane, 1-nitropentane, or 2-nitropropane (PubMed:25002579).[1] [2] [3]
See Also
References
- ↑ Nishino SF, Shin KA, Payne RB, Spain JC. Growth of bacteria on 3-nitropropionic acid as a sole source of carbon, nitrogen, and energy. Appl Environ Microbiol. 2010 Jun;76(11):3590-8. PMID:20382807 doi:10.1128/AEM.00267-10
- ↑ Salvi F, Agniswamy J, Yuan H, Vercammen K, Pelicaen R, Cornelis P, Spain J, Weber IT, Gadda G. The Combined Structural and Kinetic Characterization of a Bacterial Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1 Establishes NMO Class I and II. J Biol Chem. 2014 Jul 7. pii: jbc.M114.577791. PMID:25002579 doi:http://dx.doi.org/10.1074/jbc.M114.577791
- ↑ Vercammen K, Wei Q, Charlier D, Dötsch A, Haüssler S, Schulz S, Salvi F, Gadda G, Spain J, Rybtke ML, Tolker-Nielsen T, Dingemans J, Ye L, Cornelis P. Pseudomonas aeruginosa LysR PA4203 regulator NmoR acts as a repressor of the PA4202 nmoA gene, encoding a nitronate monooxygenase. J Bacteriol. 2015 Mar;197(6):1026-39. PMID:25384477 doi:10.1128/JB.01991-14
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