4rud

From Proteopedia

Crystal structure of a three finger toxin

Structural highlights

4rud is a 2 chain structure with sequence from Micrurus fulvius. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.95Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

3SXB_MICFL Postsynaptic neurotoxin that produces potent, and completely reversible, postsynaptic neuromuscular blockade, as well as broad spectrum inhibition of human muscle and neuronal nicotinic acetylcholine receptors (nAChRs). Inhibition is potent or moderate, depending on the receptor (alpha-1-beta-1-delta-epsilon/CHRNA1-CHRNB1-CHRND-CHRNE (IC(50)=2.56 uM), alpha-4-beta-2/CHRNA4-CHRNB2 (IC(50)=1.8 uM), alpha-7/CHRNA7 (IC(50)=7 uM), and alpha-3-beta-2/CHRNA3-CHRNB2 (IC(50)=12.6 uM)). Acts as a competitive antagonist of ACh. Binds to chicken muscle-type nicotinic acetylcholine receptor (AChR) with high potency compared with the cloned human receptor. Unlike short-chain alpha-3FTxs that only bind to muscle nAChRs, this toxin utilizes dimerization to expand its pharmacological targets to block neuronal nAChRs.[UniProtKB:U3FAE1]

Publication Abstract from PubMed

BACKGROUND AND PURPOSE: Animal toxins have contributed significantly to our understanding of the neurobiology of receptors and ion channels. We studied the venom of the coral snake Micrurus fulvius fulvius and identified and characterised the structure and pharmacology of a new homodimeric neurotoxin, fulditoxin, that exhibited novel pharmacology at nicotinic acetylcholine receptors (nAChRs). EXPERIMENTAL APPROACH: Fulditoxin was isolated by chromatography, chemically synthesised, its structure determined by X-ray crystallography, and its pharmacological actions on nAChRs characterised by organ bath assays and two-electrode voltage clamp electrophysiology. KEY RESULTS: Fulditoxin's distinct 1.95A quaternary structure revealed two short-chain three-finger alpha-neurotoxins (alpha-3FNTxs) non-covalently bound by hydrophobic interactions; and an ability to bind metal and form tetrameric complexes, not reported previously for three-finger proteins. Although fulditoxin lacked all conserved amino acids canonically important for inhibiting nAChRs, it produced postsynaptic neuromuscular blockade of chick muscle at nanomolar concentrations, comparable to the prototypical alpha-bungarotoxin. This neuromuscular blockade was completely reversible, which is unusual for snake alpha-3FNTxs. Fulditoxin, therefore, interacts with nAChRs by utilizing an alternate pharmacophore. Unlike short-chain alpha-3FNTxs that bind only to muscle nAChRs, fulditoxin utilises dimerization to expand its pharmacological targets to include human neuronal alpha4beta2, alpha7 and alpha3beta2 nAChRs which it blocked with IC50 values of 1.8, 7, and 12 muM, respectively. CONCLUSIONS AND IMPLICATIONS: Based on its distinct quaternary structure and unusual pharmacology, we named this new class of dimeric Micrurus neurotoxins represented by fulditoxin as Sigma-neurotoxins, which offers greater insight into understanding the interactions between nAChRs and peptide antagonists.

Fulditoxin, representing a new class of dimeric snake toxins, defines novel pharmacology at nicotinic acetylcholine receptors.,Foo CS, Jobichen C, Hassan-Puttaswamy V, Dekan Z, Tae HS, Bertrand D, Adams DJ, Alewood PF, Sivaraman J, Nirthanan S, Kini RM Br J Pharmacol. 2019 Dec 26. doi: 10.1111/bph.14954. PMID:31877243^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Foo CS, Jobichen C, Hassan-Puttaswamy V, Dekan Z, Tae HS, Bertrand D, Adams DJ, Alewood PF, Sivaraman J, Nirthanan S, Kini RM. Fulditoxin, representing a new class of dimeric snake toxins, defines novel pharmacology at nicotinic acetylcholine receptors. Br J Pharmacol. 2019 Dec 26. doi: 10.1111/bph.14954. PMID:31877243 doi:http://dx.doi.org/10.1111/bph.14954