4v64

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Crystal structure of the bacterial ribosome from Escherichia coli in complex with hygromycin B.

Structural highlights

4v64 is a 20 chain structure with sequence from Escherichia coli. This structure supersedes the now removed PDB entries 3df1, 3df2, 3df3 and 3df4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.5Å
Ligands:HYG, MG, ZN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL20_ECOLI One of the primary rRNA binding proteins, it binds close to the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly.[HAMAP-Rule:MF_00382]

Publication Abstract from PubMed

Aminoglycosides are one of the most widely used and clinically important classes of antibiotics that target the ribosome. Hygromycin B is an atypical aminoglycoside antibiotic with unique structural and functional properties. Here we describe the structure of the intact Escherichia coli 70S ribosome in complex with hygromycin B. The antibiotic binds to the mRNA decoding center in the small (30S) ribosomal subunit of the 70S ribosome and induces a localized conformational change, in contrast to its effects observed in the structure of the isolated 30S ribosomal subunit in complex with the drug. The conformational change in the ribosome caused by hygromycin B binding differs from that induced by other aminoglycosides. Also, in contrast to other aminoglycosides, hygromycin B potently inhibits spontaneous reverse translocation of tRNAs and mRNA on the ribosome in vitro. These structural and biochemical results help to explain the unique mode of translation inhibition by hygromycin B.

Structural basis for hygromycin B inhibition of protein biosynthesis.,Borovinskaya MA, Shoji S, Fredrick K, Cate JH RNA. 2008 Aug;14(8):1590-9. Epub 2008 Jun 20. PMID:18567815[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
15 reviews cite this structure
Ribosome-targeting antibiotics and mechanisms of bacterial resistance.
Wilson et al. (2014)
14 more
56 other articles
No citations found

See Also

References

  1. ↑ Borovinskaya MA, Shoji S, Fredrick K, Cate JH. Structural basis for hygromycin B inhibition of protein biosynthesis. RNA. 2008 Aug;14(8):1590-9. Epub 2008 Jun 20. PMID:18567815 doi:10.1261/rna.1076908

Contents


PDB ID 4v64

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