4v8n

From Proteopedia

Jump to: navigation, search

The crystal structure of agmatidine tRNA-Ile2 bound to the 70S ribosome in the A and P site.

Structural highlights

4v8n is a 20 chain structure with sequence from Thermus thermophilus HB8. This structure supersedes the now removed PDB entries 3zn7, 3zn9, 3znd and 3zne. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.1Å
Ligands:AG9, ZN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RS4_THET8 One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the body and platform of the 30S subunit. Binds mRNA in the 70S ribosome, positioning it for translation.[HAMAP-Rule:MF_01306_B]

Publication Abstract from PubMed

Decoding of the AUA isoleucine codon in bacteria and archaea requires modification of a C in the anticodon wobble position of the isoleucine tRNA. Here, we report the crystal structure of the archaeal tRNA2Ile, which contains the modification agmatidine in its anticodon, in complex with the AUA codon on the 70S ribosome. The structure illustrates how agmatidine confers codon specificity for AUA over AUG.

The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome.,Voorhees RM, Mandal D, Neubauer C, Kohrer C, Rajbhandary UL, Ramakrishnan V Nat Struct Mol Biol. 2013 Mar 31. doi: 10.1038/nsmb.2545. PMID:23542153[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Loading citation details..
Citations
13 reviews cite this structure
The Importance of Being Modified: The Role of RNA Modifications in Translational Fidelity.
Agris et al. (2017)
12 more
18 other articles
No citations found

See Also

References

  1. ↑ Voorhees RM, Mandal D, Neubauer C, Kohrer C, Rajbhandary UL, Ramakrishnan V. The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome. Nat Struct Mol Biol. 2013 Mar 31. doi: 10.1038/nsmb.2545. PMID:23542153 doi:10.1038/nsmb.2545

Contents


PDB ID 4v8n

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/4v8n"
Personal tools