4v9a

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Crystal Structure of the 70S ribosome with tetracycline.

Structural highlights

4v9a is a 20 chain structure with sequence from Thermus thermophilus HB8. This structure supersedes the now removed PDB entries 4g5k, 4g5l, 4g5m and 4g5n. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.2999Å
Ligands:MG, TAC, ZN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL2_THET8 One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial (By similarity). Makes several contacts with the 16S rRNA (forming bridge B7b) in the 70S ribosome.[HAMAP-Rule:MF_01320_B]

Publication Abstract from PubMed

Here we present an X-ray crystallography structure of the clinically relevant tigecycline antibiotic bound to the 70S ribosome. Our structural and biochemical analysis indicate that the enhanced potency of tigecycline results from a stacking interaction with nucleobase C1054 within the decoding site of the ribosome. Single-molecule fluorescence resonance energy transfer studies reveal that, during decoding, tigecycline inhibits the initial codon recognition step of tRNA accommodation and prevents rescue by the tetracycline-resistance protein TetM.

Structural basis for potent inhibitory activity of the antibiotic tigecycline during protein synthesis.,Jenner L, Starosta AL, Terry DS, Mikolajka A, Filonava L, Yusupov M, Blanchard SC, Wilson DN, Yusupova G Proc Natl Acad Sci U S A. 2013 Feb 19. PMID:23431179[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
27 reviews cite this structure
Ribosome-targeting antibiotics and mechanisms of bacterial resistance.
Wilson et al. (2014)
26 more
76 other articles
No citations found

See Also

References

  1. ↑ Jenner L, Starosta AL, Terry DS, Mikolajka A, Filonava L, Yusupov M, Blanchard SC, Wilson DN, Yusupova G. Structural basis for potent inhibitory activity of the antibiotic tigecycline during protein synthesis. Proc Natl Acad Sci U S A. 2013 Feb 19. PMID:23431179 doi:http://dx.doi.org/10.1073/pnas.1216691110

Contents


PDB ID 4v9a

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