5c0m

From Proteopedia

Jump to: navigation, search

Crystal structure of SGF29 tandem tudor domain in complex with a Carba containing peptide

Structural highlights

5c0m is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Ligands:4WQ, GOL, SO4, UNX
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SGF29_HUMAN Involved in transcriptional regulation, through association with histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes. Specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3). In the SAGA-type complexes, required to recruit complexes to H3K4me. May be involved in MYC-mediated oncogenic transformation.[1]

Publication Abstract from PubMed

A large number of structurally diverse epigenetic reader proteins specifically recognize methylated lysine residues on histone proteins. Here we describe comparative thermodynamic, structural and computational studies on recognition of the positively charged natural trimethyllysine and its neutral analogues by reader proteins. This work provides experimental and theoretical evidence that reader proteins predominantly recognize trimethyllysine via a combination of favourable cation-pi interactions and the release of the high-energy water molecules that occupy the aromatic cage of reader proteins on the association with the trimethyllysine side chain. These results have implications in rational drug design by specifically targeting the aromatic cage of readers of trimethyllysine.

Chemical basis for the recognition of trimethyllysine by epigenetic reader proteins.,Kamps JJ, Huang J, Poater J, Xu C, Pieters BJ, Dong A, Min J, Sherman W, Beuming T, Matthias Bickelhaupt F, Li H, Mecinovic J Nat Commun. 2015 Nov 18;6:8911. doi: 10.1038/ncomms9911. PMID:26578293[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Loading citation details..
Citations
9 reviews cite this structure
Chemical and Biochemical Perspectives of Protein Lysine Methylation.
Luo et al. (2018)
8 more
31 other articles
No citations found

See Also

References

  1. ↑ Guelman S, Kozuka K, Mao Y, Pham V, Solloway MJ, Wang J, Wu J, Lill JR, Zha J. The double-histone-acetyltransferase complex ATAC is essential for mammalian development. Mol Cell Biol. 2009 Mar;29(5):1176-88. doi: 10.1128/MCB.01599-08. Epub 2008 Dec, 22. PMID:19103755 doi:10.1128/MCB.01599-08
  2. ↑ Kamps JJ, Huang J, Poater J, Xu C, Pieters BJ, Dong A, Min J, Sherman W, Beuming T, Matthias Bickelhaupt F, Li H, Mecinovic J. Chemical basis for the recognition of trimethyllysine by epigenetic reader proteins. Nat Commun. 2015 Nov 18;6:8911. doi: 10.1038/ncomms9911. PMID:26578293 doi:http://dx.doi.org/10.1038/ncomms9911

Contents


PDB ID 5c0m

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/5c0m"
Personal tools