5jyg

Structural highlights

Function

MAMK_MAGSA Protein with ATPase activity which forms dynamic cytoplasmic filaments (probably with paralog MamK-like) that organize magnetosomes into long chains running parallel to the long axis of the cell (Probable) (PubMed:21883528, PubMed:28790202, PubMed:23204522, PubMed:24957623). Turnover of MamK filaments is probably promoted by MamK-like, which provides a monomer pool (PubMed:24957623). Forms twisted filaments in the presence of ATP or GTP (PubMed:20161777, PubMed:23204522, PubMed:27391173). Serves to close gaps between magnetosomes in the chain (PubMed:26884433). Interaction with MCP10 is involved in controlling the response to magnetic fields, possibly by controlling flagellar rotation (PubMed:20471399). Expression in E.coli yields a filament in the cell's longitudinal axis; the protein nucleates at several sites and one extremity of the filament is located at the cell pole (PubMed:17085581, PubMed:20161777).^{[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]}

References

1. ↑ Pradel N, Santini CL, Bernadac A, Fukumori Y, Wu LF. Biogenesis of actin-like bacterial cytoskeletal filaments destined for positioning prokaryotic magnetic organelles. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17485-9. PMID:17085581 doi:10.1073/pnas.0603760103
2. ↑ Rioux JB, Philippe N, Pereira S, Pignol D, Wu LF, Ginet N. A second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island. PLoS One. 2010 Feb 10;5(2):e9151. PMID:20161777 doi:10.1371/journal.pone.0009151
3. ↑ Philippe N, Wu LF. An MCP-like protein interacts with the MamK cytoskeleton and is involved in magnetotaxis in Magnetospirillum magneticum AMB-1. J Mol Biol. 2010 Jul 16;400(3):309-22. PMID:20471399 doi:10.1016/j.jmb.2010.05.011
4. ↑ Draper O, Byrne ME, Li Z, Keyhani S, Barrozo JC, Jensen G, Komeili A. MamK, a bacterial actin, forms dynamic filaments in vivo that are regulated by the acidic proteins MamJ and LimJ. Mol Microbiol. 2011 Oct;82(2):342-54. PMID:21883528 doi:10.1111/j.1365-2958.2011.07815.x
5. ↑ Ozyamak E, Kollman J, Agard DA, Komeili A. The bacterial actin MamK: in vitro assembly behavior and filament architecture. J Biol Chem. 2013 Feb 8;288(6):4265-77. PMID:23204522 doi:10.1074/jbc.M112.417030
6. ↑ Abreu N, Mannoubi S, Ozyamak E, Pignol D, Ginet N, Komeili A. Interplay between two bacterial actin homologs, MamK and MamK-Like, is required for the alignment of magnetosome organelles in Magnetospirillum magneticum AMB-1. J Bacteriol. 2014 Sep;196(17):3111-21. PMID:24957623 doi:10.1128/JB.01674-14
7. ↑ Cornejo E, Subramanian P, Li Z, Jensen GJ, Komeili A. Dynamic Remodeling of the Magnetosome Membrane Is Triggered by the Initiation of Biomineralization. mBio. 2016 Feb 16;7(1):e01898-15. PMID:26884433 doi:10.1128/mBio.01898-15
8. ↑ Bergeron JR, Hutto R, Ozyamak E, Hom N, Hansen J, Draper O, Byrne ME, Keyhani S, Komeili A, Kollman JM. Structure of the Magnetosome-associated actin-like MamK filament at sub-nanometer resolution. Protein Sci. 2016 Jul 8. doi: 10.1002/pro.2979. PMID:27391173 doi:http://dx.doi.org/10.1002/pro.2979
9. ↑ Taoka A, Kiyokawa A, Uesugi C, Kikuchi Y, Oestreicher Z, Morii K, Eguchi Y, Fukumori Y. Tethered Magnets Are the Key to Magnetotaxis: Direct Observations of Magnetospirillum magneticum AMB-1 Show that MamK Distributes Magnetosome Organelles Equally to Daughter Cells. mBio. 2017 Aug 8;8(4):e00679-17. PMID:28790202 doi:10.1128/mBio.00679-17
10. ↑ Komeili A, Li Z, Newman DK, Jensen GJ. Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK. Science. 2006 Jan 13;311(5758):242-5. PMID:16373532 doi:10.1126/science.1123231