Structural highlights
Function
[PPA1_PEDAC] Bactericidal activity (effective inhibitor of L.monocytogenes).
Publication Abstract from PubMed
The antimicrobial peptide pediocin PA-1 is a class IIa bacteriocin that inhibits several clinically relevant pathogens including Listeria spp. Here we report the synthesis and characterization of whole pediocin PA-1 and novel analogs thereof using a combination of solid- and solution-phase strategies to overcome difficulties due to instability and undesired reactions. Pediocin PA-1 thus synthesized was a potent inhibitor of Listeria monocytogenes (MIC = 6.8 nM), similar to the bacteriocin produced naturally by Pediococcus acidilactici. Of particular interest is that linear analogs lacking both of the disulfide bridges characterizing pediocin PA-1 were as potent. One linear analog was also a strong inhibitor of Clostridium perfringens, another important food-borne pathogen. These results are discussed in light of conformational information derived from circular dichroism, solution NMR spectroscopy and structure-activity relationship studies.
Synthesis, antimicrobial activity and conformational analysis of the class IIa bacteriocin pediocin PA-1 and analogs thereof.,Bedard F, Hammami R, Zirah S, Rebuffat S, Fliss I, Biron E Sci Rep. 2018 Jun 13;8(1):9029. doi: 10.1038/s41598-018-27225-3. PMID:29899567[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bedard F, Hammami R, Zirah S, Rebuffat S, Fliss I, Biron E. Synthesis, antimicrobial activity and conformational analysis of the class IIa bacteriocin pediocin PA-1 and analogs thereof. Sci Rep. 2018 Jun 13;8(1):9029. doi: 10.1038/s41598-018-27225-3. PMID:29899567 doi:http://dx.doi.org/10.1038/s41598-018-27225-3
