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5yjg

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Structural insights into periostin functions

Structural highlights

5yjg is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.399Å
Ligands:	, , , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

POSTN_HUMAN Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).[UniProtKB:Q62009]^[1]

Publication Abstract from PubMed

Human periostin plays a multifaceted role in remodeling the extracellular matrix milieu by interacting with other proteins and itself in both a heterophilic and homophilic manner. However, the structural mechanism for its extensive interactions has remained elusive. Here, we report the crystal structures of human periostin (EMI-Fas1(I-)(IV) ) and its Cys60Ala mutant. In combination with multi-angle light-scattering analysis and biochemical assays, the crystal structures reveal that periostin mainly exists as a dimer in solution and its homophilic interaction is mainly mediated by the EMI domain. Furthermore, Cys60 undergoes cysteinylation as confirmed by mass spectroscopy, and this site hardly affects the homophilic interaction. Also, the structures yield insights into how periostin forms heterophilic interactions with other proteins under physiological or pathological conditions.

Structural characterizations of human periostin dimerization and cysteinylation.,Liu J, Zhang J, Xu F, Lin Z, Li Z, Liu H FEBS Lett. 2018 Jun;592(11):1789-1803. doi: 10.1002/1873-3468.13091. Epub 2018, May 21. PMID:29754429^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gillan L, Matei D, Fishman DA, Gerbin CS, Karlan BY, Chang DD. Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins and promotes cell motility. Cancer Res. 2002 Sep 15;62(18):5358-64. PMID:12235007
↑ Liu J, Zhang J, Xu F, Lin Z, Li Z, Liu H. Structural characterizations of human periostin dimerization and cysteinylation. FEBS Lett. 2018 Jun;592(11):1789-1803. doi: 10.1002/1873-3468.13091. Epub 2018, May 21. PMID:29754429 doi:http://dx.doi.org/10.1002/1873-3468.13091