Structural highlights
Function
[ABCF1_HUMAN] Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.[1]
Publication Abstract from PubMed
Gene translation requires the correct selection of start codon AUG in mRNA. ATP-binding cassette subfamily F member 1 (ABCF1) plays a key role in the accuracy of start codon selection. However, the function of human ABCF1 is not clearly understood. Here, we solve the crystal structure of an ATP-bound wild-type human ABCF1 at 2.3-A resolution. The comparative studies indicate that the structure is in a pre-activation intermediate conformation. This conformation is stabilized by the interaction between ATP and protein. Thus, we propose that this conformation is an important step in the activation of ABCF1. This study extends our understanding of ABC (ATP-binding cassette) protein activation at the molecular level.
Crystal Structure of ATP-Bound Human ABCF1 Demonstrates a Unique Conformation of ABC Proteins.,Qu L, Jiang Y, Cheng C, Wu D, Meng B, Chen Z, Zhu Y, Shaw N, Ouyang S, Liu ZJ Structure. 2018 Jun 12. pii: S0969-2126(18)30206-5. doi:, 10.1016/j.str.2018.05.019. PMID:30017566[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Paytubi S, Wang X, Lam YW, Izquierdo L, Hunter MJ, Jan E, Hundal HS, Proud CG. ABC50 promotes translation initiation in mammalian cells. J Biol Chem. 2009 Sep 4;284(36):24061-73. doi: 10.1074/jbc.M109.031625. Epub 2009, Jul 1. PMID:19570978 doi:http://dx.doi.org/10.1074/jbc.M109.031625
- ↑ Qu L, Jiang Y, Cheng C, Wu D, Meng B, Chen Z, Zhu Y, Shaw N, Ouyang S, Liu ZJ. Crystal Structure of ATP-Bound Human ABCF1 Demonstrates a Unique Conformation of ABC Proteins. Structure. 2018 Jun 12. pii: S0969-2126(18)30206-5. doi:, 10.1016/j.str.2018.05.019. PMID:30017566 doi:http://dx.doi.org/10.1016/j.str.2018.05.019
