6b4v

From Proteopedia

Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome

Structural highlights

6b4v is a 22 chain structure with sequence from Escherichia coli K-12 and Thermus thermophilus HB27. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.4Å
Ligands:	0TD, 2MA, 2MG, 2MU, 4OC, 4SU, 5MC, 5MU, 7MG, BLS, M2G, MA6, MG, OMG, PSU, UR3, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL9_THET2 Binds to the 23S rRNA (By similarity).

Publication Abstract from PubMed

Understanding the mechanisms of inhibitors of translation termination may inform development of new antibacterials and therapeutics for premature termination diseases. We report the crystal structure of the potent termination inhibitor blasticidin S bound to the ribosomal 70S*release factor 1 (RF1) termination complex. Blasticidin S shifts the catalytic domain 3 of RF1 and restructures the peptidyl transferase center. Universally conserved uridine 2585 in the peptidyl transferase center occludes the catalytic backbone of the GGQ motif of RF1, explaining the structural mechanism of inhibition. Rearrangement of domain 3 relative to the codon-recognition domain 2 provides insight into the dynamics of RF1 implicated in termination accuracy.

Mechanism of Inhibition of Translation Termination by Blasticidin S.,Svidritskiy E, Korostelev AA J Mol Biol. 2018 Feb 2. pii: S0022-2836(18)30010-X. doi:, 10.1016/j.jmb.2018.01.007. PMID:29366636^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations

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References

↑ Svidritskiy E, Korostelev AA. Mechanism of Inhibition of Translation Termination by Blasticidin S. J Mol Biol. 2018 Feb 2. pii: S0022-2836(18)30010-X. doi:, 10.1016/j.jmb.2018.01.007. PMID:29366636 doi:http://dx.doi.org/10.1016/j.jmb.2018.01.007

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

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PDB ID 6b4v

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Categories: Escherichia coli K-12 | Large Structures | Thermus thermophilus HB27 | Korostelev AA | Svidritskiy E

6b4v

From Proteopedia

Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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