6cr0

Structural highlights

Function

HLNO_SHIS7 Involved in the degradation of L-nicotine (PubMed:25002425). Catalyzes the oxidation of (S)-6-hydroxynicotine (6-hydroxy-L-nicotine) to 6-hydroxypseudooxynicotine (PubMed:25002425). Oxidation of the pyrrolidine ring of (S)-6-hydroxynicotine leads to the formation of the optically inactive 6-hydroxy-N-methylmyosmine, which hydrolyzes spontaneously to 6-hydroxypseudooxynicotine (PubMed:25002425). Acts with absolute stereospecificity on the L-form of 6-hydroxynicotine (PubMed:25002425). Also involved in the degradation of nornicotine, and catalyzes the oxidation of 6-hydroxynornicotine to 6-hydroxymyosmine, which hydrolyzes to 6-hydroxypseudooxynornicotine (PubMed:27568381). In vitro, converts (S)-nicotine into N-methylmyosmine, which spontaneously hydrolyzes spontaneously into pseudooxynicotine, but catalytic efficiency is about 1900-fold higher with (S)-6-hydroxynicotine (PubMed:25002425).^{[1] [2]}

References

1. ↑ Qiu J, Wei Y, Ma Y, Wen R, Wen Y, Liu W. A novel (S)-6-hydroxynicotine oxidase gene from Shinella sp. strain HZN7. Appl Environ Microbiol. 2014 Sep;80(18):5552-60. PMID:25002425 doi:10.1128/AEM.01312-14
2. ↑ Qiu J, Li N, Lu Z, Yang Y, Ma Y, Niu L, He J, Liu W. Conversion of nornicotine to 6-hydroxy-nornicotine and 6-hydroxy-myosmine by Shinella sp. strain HZN7. Appl Microbiol Biotechnol. 2016 Dec;100(23):10019-10029. PMID:27568381 doi:10.1007/s00253-016-7805-0