6gbi
From Proteopedia
Wnt signalling
Structural highlights
Publication Abstract from PubMedLYPD6 is a Wnt signaling enhancer that promotes phosphorylation of the Wnt co-receptor LRP6 (low-density lipoprotein receptor-related protein 6). It also binds the nicotinic acetylcholine receptor (nAChR). We report here the 1.25 A resolution structure of the LYPD6 extracellular Ly6/uPAR (LU) domain and map its interaction with LRP6 by mutagenesis and surface plasmon resonance (SPR). The LYPD6LU structure reveals a "tri-fingered protein domain" fold with the middle fingertip of the bearing a "NxI" motif, a tripeptide motif associated with LRP5/6 binding by Wnt inhibitors. Of the Ly6 protein family members, only LYPD6 has an NxI motif. Since mutations in the LYPD6 NxI motif abolish or severely reduce interaction with LRP6, our results indicate its key role in the interaction of LYPD6 with LRP6. This article is protected by copyright. All rights reserved. Structure of the Wnt signaling enhancer LYPD6 and its interactions with the Wnt co-receptor LRP6.,Zhao Y, Ren J, Lu W, Harlos K, Jones EY FEBS Lett. 2018 Aug 1. doi: 10.1002/1873-3468.13212. PMID:30069874[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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