6iei

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Loop deletion and proline insertion mutant (deleting six residues and inserted five proline residues)

Structural highlights

6iei is a 1 chain structure with sequence from Borreliella burgdorferi B31. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

OSPA_BORBU

Publication Abstract from PubMed

In "domain-swapping," proteins mutually interconvert structural elements to form a dimer/oligomer. Engineering this process by design is important for creating a higher order protein assembly with minimal modification. Herein, we show a simple design strategy for domain-swapping formation by loop deletion and insertion of a polyproline rod. Crystal structures revealed the formation of the domain-swapped dimers and the polyproline portion formed a polyproline II (PPII) structure. Small-angle x-ray scattering (SAXS) demonstrated that an extended orientation of domain-swapped dimer was retained in the solution. We found that a multiple of three of inserting proline residue is favored for domain-swapping because of the helical nature of PPII. The rigid nature of the polyproline rod enables precise control of the interdomain distance and orientation.

Domain-swapping design by poly-proline rod insertion.,Shiga S, Yamanaka M, Fujiwara W, Hirota S, Goda S, Makabe K Chembiochem. 2019 May 15. doi: 10.1002/cbic.201900179. PMID:31094059[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
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Experimental and theoretical study on converting myoglobin into a stable domain-swapped dimer by utilizing a tight hydrogen bond network at the hinge region.
Xie et al. (2021)
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1 other articles
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See Also

References

  1. ↑ Shiga S, Yamanaka M, Fujiwara W, Hirota S, Goda S, Makabe K. Domain-swapping design by poly-proline rod insertion. Chembiochem. 2019 May 15. doi: 10.1002/cbic.201900179. PMID:31094059 doi:http://dx.doi.org/10.1002/cbic.201900179

Contents


PDB ID 6iei

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OCA

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