6ifm

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Crystal structure of DNA bound VapBC from Salmonella typhimurium

Structural highlights

6ifm is a 10 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.804Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VAPC_SALTY Toxic component of a type II toxin-antitoxin (TA) system. A site-specific tRNA-(fMet) endonuclease, it cleaves both charged and uncharged tRNA-(fMet) between positions 38 and 39 at the anticodon stem-loop boundary. Does not cleave tRNA(Met), tRNA(Arg2), tRNA(His), tRNA(Leu), tRNA(Phe) tRNA(Thr1), tRNA(Tyr) or tRNA(Val). Overexpression in E.coli inhibits translation, leads to loss of cell growth and degradation of tRNA(fMet), these effects are neutralized by expression of cognate antitoxin VapB. Expression also activates translation initiation at correctly positioned elongator codons (AAA and to a lesser extent AAG). Ectopic overexpression in E.coli induces the YoeB toxin, but this is not the cause of VapC toxicity.

See Also

Contents


PDB ID 6ifm

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