| Structural highlights
6jau is a 2 chain structure with sequence from Pseae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , , |
| Gene: | mucB, algN, PA0764 (PSEAE), mucA, PA0763 (PSEAE) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[MUCB_PSEAE] Negative regulator of the sigma factor AlgU. Plays a role in the differentiation of P.aeruginosa into the alginate-producing form. Inactivation of mucB causes conversion to mucoidy. [MUCA_PSEAE] Negative regulator of the sigma factor AlgU. Plays a role in the differentiation of P.aeruginosa into the alginate-producing form. Inactivation of mucA causes a switch from the non-mucoid to mucoid state resulting in constitutive expression of alginate biosynthetic genes.
Publication Abstract from PubMed
MucA and MucB are critical negative modulators of sigma factor AlgU and regulate the mucoid conversion of Pseudomonas aeruginosa. Previous studies have revealed that lipid signals antagonize MucA-MucB binding. Here we report the crystal structure of MucB in complex with the periplasmic domain of MucA and polyethylene glycol (PEG), which unveiled an intermediate state preceding the MucA-MucB dissociation. Based on the biochemical experiments, the aliphatic side chain with a polar group was found to be of primary importance for inducing MucA cleavage. These results provide evidence that the hydrophobic cavity of MucB is a primary site for sensing lipid molecules and illustrates the detailed control of conformational switching within MucA-MucB in response to lipophilic effectors.
Molecular basis of the lipid-induced MucA-MucB dissociation in Pseudomonas aeruginosa.,Li T, He L, Li C, Kang M, Song Y, Zhu Y, Shen Y, Zhao N, Zhao C, Yang J, Huang Q, Mou X, Tong A, Yang J, Wang Z, Ji C, Li H, Tang H, Bao R Commun Biol. 2020 Aug 3;3(1):418. doi: 10.1038/s42003-020-01147-1. PMID:32747658[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li T, He L, Li C, Kang M, Song Y, Zhu Y, Shen Y, Zhao N, Zhao C, Yang J, Huang Q, Mou X, Tong A, Yang J, Wang Z, Ji C, Li H, Tang H, Bao R. Molecular basis of the lipid-induced MucA-MucB dissociation in Pseudomonas aeruginosa. Commun Biol. 2020 Aug 3;3(1):418. doi: 10.1038/s42003-020-01147-1. PMID:32747658 doi:http://dx.doi.org/10.1038/s42003-020-01147-1
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