| Structural highlights
Function
PIEZ2_MOUSE Component of a mechanosensitive channel required for rapidly adapting mechanically activated (MA) currents (PubMed:20813920, PubMed:24717433). Required for Merkel-cell mechanotransduction (PubMed:24717433). Plays a major role in light-touch mechanosensation (PubMed:25471886).[1] [2] [3]
Publication Abstract from PubMed
PIEZO2 is a mechanosensitive cation channel that has a key role in sensing touch, tactile pain, breathing and blood pressure. Here we describe the cryo-electron microscopy structure of mouse PIEZO2, which is a three-bladed, propeller-like trimer that comprises 114 transmembrane helices (38 per protomer). Transmembrane helices 1-36 (TM1-36) are folded into nine tandem units of four transmembrane helices each to form the unusual non-planar blades. The three blades are collectively curved into a nano-dome of 28-nm diameter and 10-nm depth, with an extracellular cap-like structure embedded in the centre and a 9-nm-long intracellular beam connecting to the central pore. TM38 and the C-terminal domain are surrounded by the anchor domain and TM37, and enclose the central pore with both transmembrane and cytoplasmic constriction sites. Structural comparison between PIEZO2 and its homologue PIEZO1 reveals that the transmembrane constriction site might act as a transmembrane gate that is controlled by the cap domain. Together, our studies provide insights into the structure and mechanogating mechanism of Piezo channels.
Structure and mechanogating of the mammalian tactile channel PIEZO2.,Wang L, Zhou H, Zhang M, Liu W, Deng T, Zhao Q, Li Y, Lei J, Li X, Xiao B Nature. 2019 Sep;573(7773):225-229. doi: 10.1038/s41586-019-1505-8. Epub 2019 Aug, 21. PMID:31435011[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, Dubin AE, Patapoutian A. Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. Science. 2010 Oct 1;330(6000):55-60. doi: 10.1126/science.1193270. Epub 2010 Sep , 2. PMID:20813920 doi:http://dx.doi.org/10.1126/science.1193270
- ↑ Woo SH, Ranade S, Weyer AD, Dubin AE, Baba Y, Qiu Z, Petrus M, Miyamoto T, Reddy K, Lumpkin EA, Stucky CL, Patapoutian A. Piezo2 is required for Merkel-cell mechanotransduction. Nature. 2014 May 29;509(7502):622-6. doi: 10.1038/nature13251. Epub 2014 Apr 6. PMID:24717433 doi:http://dx.doi.org/10.1038/nature13251
- ↑ Ranade SS, Woo SH, Dubin AE, Moshourab RA, Wetzel C, Petrus M, Mathur J, Begay V, Coste B, Mainquist J, Wilson AJ, Francisco AG, Reddy K, Qiu Z, Wood JN, Lewin GR, Patapoutian A. Piezo2 is the major transducer of mechanical forces for touch sensation in mice. Nature. 2014 Dec 4;516(7529):121-5. doi: 10.1038/nature13980. PMID:25471886 doi:http://dx.doi.org/10.1038/nature13980
- ↑ Wang L, Zhou H, Zhang M, Liu W, Deng T, Zhao Q, Li Y, Lei J, Li X, Xiao B. Structure and mechanogating of the mammalian tactile channel PIEZO2. Nature. 2019 Sep;573(7773):225-229. doi: 10.1038/s41586-019-1505-8. Epub 2019 Aug, 21. PMID:31435011 doi:http://dx.doi.org/10.1038/s41586-019-1505-8
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