6ncl

From Proteopedia

Near-atomic structure of icosahedrally averaged PBCV-1 capsid

6ncl, resolution 3.50Å

Structural highlights

6ncl is a 22 chain structure with sequence from Paramecium bursaria Chlorella virus 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.5Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

P14_PBCV1 One of the minor capsid proteins that constitute a network internal to the major capsid proteins and outside the lipid membrane (PubMed:30674888). The minor capsid protein P14 does not serve a cross-linking function between neighboring capsomers, it may play a role in the viral capsid assembly (PubMed:30674888).^[1]

Publication Abstract from PubMed

Although the nucleocytoplasmic large DNA viruses (NCLDVs) are one of the largest group of viruses that infect many eukaryotic hosts, the near-atomic resolution structures of these viruses have remained unknown. Here we describe a 3.5 A resolution icosahedrally averaged capsid structure of Paramecium bursaria chlorella virus 1 (PBCV-1). This structure consists of 5040 copies of the major capsid protein, 60 copies of the penton protein and 1800 minor capsid proteins of which there are 13 different types. The minor capsid proteins form a hexagonal network below the outer capsid shell, stabilizing the capsid by binding neighboring capsomers together. The size of the viral capsid is determined by a tape-measure, minor capsid protein of which there are 60 copies in the virion. Homologs of the tape-measure protein and some of the other minor capsid proteins exist in other NCLDVs. Thus, a similar capsid assembly pathway might be used by other NCLDVs.

Near-atomic structure of a giant virus.,Fang Q, Zhu D, Agarkova I, Adhikari J, Klose T, Liu Y, Chen Z, Sun Y, Gross ML, Van Etten JL, Zhang X, Rossmann MG Nat Commun. 2019 Jan 23;10(1):388. doi: 10.1038/s41467-019-08319-6. PMID:30674888^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fang Q, Zhu D, Agarkova I, Adhikari J, Klose T, Liu Y, Chen Z, Sun Y, Gross ML, Van Etten JL, Zhang X, Rossmann MG. Near-atomic structure of a giant virus. Nat Commun. 2019 Jan 23;10(1):388. doi: 10.1038/s41467-019-08319-6. PMID:30674888 doi:http://dx.doi.org/10.1038/s41467-019-08319-6
↑ Fang Q, Zhu D, Agarkova I, Adhikari J, Klose T, Liu Y, Chen Z, Sun Y, Gross ML, Van Etten JL, Zhang X, Rossmann MG. Near-atomic structure of a giant virus. Nat Commun. 2019 Jan 23;10(1):388. doi: 10.1038/s41467-019-08319-6. PMID:30674888 doi:http://dx.doi.org/10.1038/s41467-019-08319-6