6nfv

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Structure of the KcsA-G77C mutant or the 2,4-ion bound configuration of a K+ channel selectivity filter.

Structural highlights

6nfv is a 3 chain structure with sequence from "actinomyces_lividans"_krasil'nikov_et_al._1965 "actinomyces lividans" krasil'nikov et al. 1965 and Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:1EM, F09, K
Gene:kcsA, skc1 ("Actinomyces lividans" Krasil'nikov et al. 1965)
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).[1]

Publication Abstract from PubMed

Here, we present the atomic resolution crystallographic structure, the function, and the ion-binding properties of the KcsA mutants, G77A and G77C, that stabilize the 2,4-ion-bound configuration (i.e., water, K(+), water, K(+)-ion-bound configuration) of the K(+) channel's selectivity filter. A full functional and thermodynamic characterization of the G77A mutant revealed wild-type-like ion selectivity and apparent K(+)-binding affinity, in addition to showing a lack of C-type inactivation gating and a marked reduction in its single-channel conductance. These structures validate, from a structural point of view, the notion that 2 isoenergetic ion-bound configurations coexist within a K(+) channel's selectivity filter, which fully agrees with the water-K(+)-ion-coupled transport detected by streaming potential measurements.

Structure, function, and ion-binding properties of a K(+) channel stabilized in the 2,4-ion-bound configuration.,Tilegenova C, Cortes DM, Jahovic N, Hardy E, Hariharan P, Guan L, Cuello LG Proc Natl Acad Sci U S A. 2019 Aug 6. pii: 1901888116. doi:, 10.1073/pnas.1901888116. PMID:31387976[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
1 reviews cite this structure
The Mechanism of Metal Homeostasis in Plants: A New View on the Synergistic Regulation Pathway of Membrane Proteins, Lipids and Metal Ions.
Wu et al. (2021)
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12 other articles
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See Also

References

  1. ↑ Schrempf H, Schmidt O, Kummerlen R, Hinnah S, Muller D, Betzler M, Steinkamp T, Wagner R. A prokaryotic potassium ion channel with two predicted transmembrane segments from Streptomyces lividans. EMBO J. 1995 Nov 1;14(21):5170-8. PMID:7489706
  2. ↑ Tilegenova C, Cortes DM, Jahovic N, Hardy E, Hariharan P, Guan L, Cuello LG. Structure, function, and ion-binding properties of a K(+) channel stabilized in the 2,4-ion-bound configuration. Proc Natl Acad Sci U S A. 2019 Aug 6. pii: 1901888116. doi:, 10.1073/pnas.1901888116. PMID:31387976 doi:http://dx.doi.org/10.1073/pnas.1901888116

Contents


PDB ID 6nfv

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