6s3l
From Proteopedia
Structure of the core of the flagellar export apparatus from Vibrio mimicus, the FliPQR-FlhB complex.
Structural highlights
Function[A0A1D8S9F8_VIBMI] Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.[RuleBase:RU364091] [A0A2J9UXT5_VIBMI] Plays a role in the flagellum-specific transport system.[RuleBase:RU362069] [A0A1D8S9F5_VIBMI] Role in flagellar biosynthesis.[RuleBase:RU364090] [A0A1D8S9I5_VIBMI] Role in flagellar biosynthesis.[RuleBase:RU362071] Publication Abstract from PubMedProtein secretion through type-three secretion systems (T3SS) is critical for motility and virulence of many bacteria. Proteins are transported through an export gate containing three proteins (FliPQR in flagella, SctRST in virulence systems). A fourth essential T3SS protein (FlhB/SctU) functions to "switch" secretion substrate specificity once the growing hook/needle reach their determined length. Here, we present the cryo-electron microscopy structure of an export gate containing the switch protein from a Vibrio flagellar system at 3.2 A resolution. The structure reveals that FlhB/SctU extends the helical export gate with its four predicted transmembrane helices wrapped around FliPQR/SctRST. The unusual topology of the FlhB/SctU helices creates a loop wrapped around the bottom of the closed export gate. Structure-informed mutagenesis suggests that this loop is critical in gating secretion and we propose that a series of conformational changes in the T3SS trigger opening of the gate through interactions between FlhB/SctU and FliPQR/SctRST. The substrate specificity switch FlhB assembles onto the export gate to regulate type three secretion.,Kuhlen L, Johnson S, Zeitler A, Baurle S, Deme JC, Caesar JJE, Debo R, Fisher J, Wagner S, Lea SM Nat Commun. 2020 Mar 10;11(1):1296. doi: 10.1038/s41467-020-15071-9. PMID:32157081[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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