6scg

From Proteopedia

Structure of AdhE form 1

Structural highlights

6scg is a 2 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.65Å
Ligands:	EDO, FE, MES, NAD, SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ADHE_ECOLI This enzyme has three activities: ADH, ACDH, and PFL-deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent reaction.

Publication Abstract from PubMed

The bifunctional alcohol/aldehyde dehydrogenase (AdhE) comprises both an N-terminal aldehyde dehydrogenase (AldDH) and a C-terminal alcohol dehydrogenase (ADH). In vivo, full-length AdhE oligomerizes into long oligomers known as spirosomes. However, structural analysis of AdhE is challenging owing to the heterogeneity of the spirosomes. Therefore, the domains of AdhE are best characterized separately. Here, the structure of ADH from the pathogenic Escherichia coli O157:H7 was determined to 1.65 A resolution. The dimeric crystal structure was confirmed in solution by small-angle X-ray scattering.

High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE.,Azmi L, Bragginton EC, Cadby IT, Byron O, Roe AJ, Lovering AL, Gabrielsen M Acta Crystallogr F Struct Biol Commun. 2020 Sep 1;76(Pt 9):414-421. doi:, 10.1107/S2053230X20010237. Epub 2020 Aug 19. PMID:32880589^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Loading citation details..

Citations

0 reviews cite this structure

Structures of lactaldehyde reductase, FucO, link enzyme activity to hydrogen bond networks and conformational dynamics.

Zavarise et al. (2023)

-1 more

0 other articles

No citations found

References

↑ Azmi L, Bragginton EC, Cadby IT, Byron O, Roe AJ, Lovering AL, Gabrielsen M. High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE. Acta Crystallogr F Struct Biol Commun. 2020 Sep 1;76(Pt 9):414-421. doi:, 10.1107/S2053230X20010237. Epub 2020 Aug 19. PMID:32880589 doi:http://dx.doi.org/10.1107/S2053230X20010237

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

loadScript /wiki/extensions/jsmol/j2s/core/package.js

loadScript /wiki/extensions/jsmol/j2s/core/corejmol.z.js

loadScript /wiki/extensions/jsmol/j2s/J/translation/PO.js

loadScript /wiki/extensions/jsmol/j2s/core/corescript.z.js

JSmol exec jmolApplet1 start applet null

Jmol JavaScript applet jmolApplet1__8795589033805244__ initializing

Jmol getValue debug null

Jmol getValue logLevel null

Jmol getValue allowjavascript null

AppletRegistry.checkIn(jmolApplet1__8795589033805244__)

vwrOptions:

{ "name":"jmolApplet1","applet":true,"documentBase":"https://proteopedia.org/wiki/index.php/6scg","platform":"J.awtjs2d.Platform","fullName":"jmolApplet1__8795589033805244__","codePath":"https:/wiki/extensions/jsmol/j2s/","display":"jmolApplet1_canvas2d","loadStructCallback":"manageLoadStructCallback","signedApplet":"true","appletReadyCallback":"Jmol._readyCallback","statusListener":"[J.appletjs.Jmol object]","syncId":"8795589033805244","messageCallback":"msgCb","bgcolor":"white" }

setting document base to "https://proteopedia.org/wiki/index.php/6scg"

Jmol Version: 14.31.8 2020-09-26 10:04

java.vendor: Java2Script (HTML5)

java.version: 2019-06-08 00:17:46 (JSmol/j2s)

os.name: Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)

Access: ALL

memory: 0.0/0.0

processors available: 1

useCommandThread: false

appletId:jmolApplet1 (signed)

Jmol getValue emulate null

defaults = "Jmol"

Jmol getValue boxbgcolor null

Jmol getValue bgcolor white

backgroundColor = "white"

Jmol getValue ANIMFRAMECallback null

Jmol getValue APPLETREADYCallback Jmol._readyCallback

APPLETREADYCallback = "Jmol._readyCallback"

StatusManager callback set for APPLETREADYCallback f=Jmol._readyCallback cb=APPLETREADY

Jmol getValue ATOMMOVEDCallback null

Jmol getValue AUDIOCallback null

Jmol getValue CLICKCallback null

Jmol getValue DRAGDROPCallback null

Jmol getValue ECHOCallback null

Jmol getValue ERRORCallback null

Jmol getValue EVALCallback null

Jmol getValue HOVERCallback null

Jmol getValue IMAGECallback null

Jmol getValue LOADSTRUCTCallback manageLoadStructCallback

LOADSTRUCTCallback = "manageLoadStructCallback"

StatusManager callback set for LOADSTRUCTCallback f=manageLoadStructCallback cb=LOADSTRUCT

Jmol getValue MEASURECallback null

Jmol getValue MESSAGECallback msgCb

MESSAGECallback = "msgCb"

StatusManager callback set for MESSAGECallback f=msgCb cb=MESSAGE

Jmol getValue MINIMIZATIONCallback null

Jmol getValue SERVICECallback null

Jmol getValue PICKCallback null

Jmol getValue RESIZECallback null

Jmol getValue SCRIPTCallback null

Jmol getValue SYNCCallback null

Jmol getValue STRUCTUREMODIFIEDCallback null

Jmol getValue doTranslate null

language=en_US

Jmol getValue doTranslate null

Note -- Presence of message callback disables disable translation; to enable message translation use jmolSetTranslation(true) prior to jmolApplet()

Note -- language translation disabled

Jmol getValue popupMenu null

Jmol getValue script null

Jmol getValue loadInline null

Jmol getValue load null

Jmol applet jmolApplet1__8795589033805244__ ready

script 1 started

pdbGetHeader = true

FileManager opening url https://proteopedia.org/wiki/extensions/Proteopedia/spt/functions.spt?1733933141

loadScript /wiki/extensions/jsmol/j2s/core/coretext.z.js

Loading, please wait ...

platformSpeed = 8

loadScript /wiki/extensions/jsmol/j2s/core/corescriptmath.z.js

loadScript /wiki/extensions/jsmol/j2s/J/bspt/PointIterator.js

FileManager opening url https://proteopedia.org/cgi-bin/infopdb?6scg

loadScript /wiki/extensions/jsmol/j2s/JU/JSJSONParser.js

loadScript /wiki/extensions/jsmol/j2s/JU/JSONException.js

JSON parser cannot parse NetworkError

Data for 6scg could not be found.- Using defaults.

cache cleared

[Step 1] pdb_features.ca_p_only is: integer 0

Loading, please wait ...

PDB ID 6scg

Drag the structure with the mouse to rotate

Export Animated Image

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/6scg"

Categories: Escherichia coli K-12 | Large Structures | Bragginton E | Lovering AL

6scg

From Proteopedia

Structure of AdhE form 1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox