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Publication Abstract from PubMed

Staphylococcus aureus employs the Type VIIb secretion system (T7SSb) to secrete effector proteins that either have antibacterial activities or promote bacterial persistence in mouse infection models. Here we present the crystal structure of the ATPase domain D3 of the EssC coupling protein from S. aureus USA300_FPR3757, an integral component of the T7SSb complex, resolved at 1.7 A resolution. EssC-D3 shares structural homology with FtsK/SpoIII like ATPase domains of T7SSa and T7SSb and exhibits a conserved pocket on the surface with differential amino acid composition.In T7SSa, substrate EsxB interacts with the D3 domain through this pocket. Here, we identify amino acids in this pocket that are essential for effector protein secretion in the T7SSb. Our results reveal that the adjacent ATPase domain D2 is a substrate binding site on EssC and that substrates bound to D2 require domain D3 for further transport. Point mutations in the Walker B motif of domain D3 have diametric effects on secretion activity, either abolishing or boosting it pointing to a critical role of domain D3 in the substrate transport. Finally, we identify ATPase domain D3 as a virulence determinant of S. aureus USA300_FPR3757 using an invertebrate in vivo infection model.Importance The emergence of antibiotic resistant bacteria poses a rising problem in antibiotic treatment (1). We have used the multi drug resistant S. aureus USA300_FPR3757 as model organism to study the T7SSb. Effector proteins of this system have been associated with abscess formation and bacterial persistence in mouse models (2, 3). We determined the structure of the essential ATPase domain D3 of the T7SSb at atomic resolution and validated a surface exposed pocket as a potential drug target to block secretion. Furthermore, our study provides new mechanistic insights into the T7SSb substrate transport.

Substrate interaction with the EssC coupling protein of the Type VIIb secretion system.,Mietrach N, Damian-Aparicio D, Suss BM, Lopez D, Geibel S J Bacteriol. 2020 Jan 21. pii: JB.00646-19. doi: 10.1128/JB.00646-19. PMID:31964696^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.