7cz5
From Proteopedia
Cryo-EM structure of the human growth hormone-releasing hormone receptor-Gs protein complex
Structural highlights
DiseaseGHRHR_HUMAN Isolated growth hormone deficiency type IB. The disease is caused by mutations affecting the gene represented in this entry.[1] [2] [3] FunctionGHRHR_HUMAN Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. Publication Abstract from PubMedGrowth hormone-releasing hormone (GHRH) regulates the secretion of growth hormone that virtually controls metabolism and growth of every tissue through its binding to the cognate receptor (GHRHR). Malfunction in GHRHR signaling is associated with abnormal growth, making GHRHR an attractive therapeutic target against dwarfism (e.g., isolated growth hormone deficiency, IGHD), gigantism, lipodystrophy and certain cancers. Here, we report the cryo-electron microscopy (cryo-EM) structure of the human GHRHR bound to its endogenous ligand and the stimulatory G protein at 2.6 A. This high-resolution structure reveals a characteristic hormone recognition pattern of GHRH by GHRHR, where the alpha-helical GHRH forms an extensive and continuous network of interactions involving all the extracellular loops (ECLs), all the transmembrane (TM) helices except TM4, and the extracellular domain (ECD) of GHRHR, especially the N-terminus of GHRH that engages a broad set of specific interactions with the receptor. Mutagenesis and molecular dynamics (MD) simulations uncover detailed mechanisms by which IGHD-causing mutations lead to the impairment of GHRHR function. Our findings provide insights into the molecular basis of peptide recognition and receptor activation, thereby facilitating the development of structure-based drug discovery and precision medicine. Structural basis for activation of the growth hormone-releasing hormone receptor.,Zhou F, Zhang H, Cong Z, Zhao LH, Zhou Q, Mao C, Cheng X, Shen DD, Cai X, Ma C, Wang Y, Dai A, Zhou Y, Sun W, Zhao F, Zhao S, Jiang H, Jiang Y, Yang D, Eric Xu H, Zhang Y, Wang MW Nat Commun. 2020 Oct 15;11(1):5205. doi: 10.1038/s41467-020-18945-0. PMID:33060564[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. Loading citation details.. Citations 4 reviews cite this structure No citations found See AlsoReferences
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Categories: Bos taurus | Homo sapiens | Large Structures | Rattus norvegicus | Synthetic construct | Cai X | Cheng X | Cong Z | Dai A | Jiang H | Jiang Y | Ma C | Mao C | Shen D | Sun W | Wang M | Wang Y | Xu HE | Yang D | Zhang H | Zhang Y | Zhao F | Zhao L | Zhao S | Zhou F | Zhou Q | Zhou Y