We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

7dog

From Proteopedia

Jump to: navigation, search

Crystal structure of a nuclease and capping domain of SbcD from Staphylococcus aureus

Structural highlights

7dog is a 2 chain structure with sequence from Staphylococcus aureus subsp. aureus Mu50. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.91Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SBCD_STAAM SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity (By similarity).

Publication Abstract from PubMed

The SbcCD complex is an essential component of the DNA double-strand break (DSB) repair system in bacteria. The bacterial SbcCD complex recognizes and cleaves the DNA ends in DSBs by ATP-dependent endo- and exonuclease activities as an early step of the DNA repair process. SbcD consists of nuclease, capping, and helix-loop-helix domains. Here, we present the crystal structure of a SbcD fragment from Staphylococcus aureus, which contained nuclease and capping domains, at a resolution of 2.9 A. This structure shows a dimeric assembly similar to that of the corresponding domains of SbcD from Escherichia coli. The S. aureus SbcD fragment exhibited endonuclease activities on supercoiled DNA and exonuclease activity on linear and nicked DNA. This study contributes to the understanding of the molecular basis for how bacteria can resist sterilizing treatment, causing DNA damage.

Crystal structure of the nuclease and capping domain of SbcD from Staphylococcus aureus.,Lee J, Jo I, Ahn J, Hong S, Jeong S, Kwon A, Ha NC J Microbiol. 2021 Apr 20. pii: 10.1007/s12275-021-1012-0. doi:, 10.1007/s12275-021-1012-0. PMID:33877576^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lee J, Jo I, Ahn J, Hong S, Jeong S, Kwon A, Ha NC. Crystal structure of the nuclease and capping domain of SbcD from Staphylococcus aureus. J Microbiol. 2021 Apr 20. pii: 10.1007/s12275-021-1012-0. doi:, 10.1007/s12275-021-1012-0. PMID:33877576 doi:http://dx.doi.org/10.1007/s12275-021-1012-0