7el7

Publication Abstract from PubMed

The sequence-dependent DNA secondary structures possess structure polymorphism. To date, studies on regulated ligands mainly focus on individual DNA secondary topologies, while lack focus on quadruplex-duplex hybrids (QDHs). Here, we design an organic-metal hybrid ligand L(1) Pt(dien), which matches and selectively binds one type of QDHs with lateral duplex stem-loop (QLDH) with high affinity, while shows poor affinity for other QDHs and individual G4 or duplex DNA. The solution structure of QLDH MYT1L-L(1) Pt(dien) complex was determined by NMR. The structure reveals that L(1) Pt(dien) presents a chair-type conformation, whose large aromatic "chair surface" intercalates into the G-quadruplex-duplex interface via pi-pi stacking and "backrest" platinum unit interacts with duplex region through hydrogen bonding and electrostatic interactions, showing a highly matched lock-key binding mode. Our work provided guidance for spatial matching design of selectively targeting ligands to QDH structures.

Spatial Matching Selectivity and Solution Structure of Organic-Metal Hybrid to Quadruplex-Duplex Hybrid.,Liu LY, Wang KN, Liu W, Zeng YL, Hou MX, Yang J, Mao ZW Angew Chem Int Ed Engl. 2021 Sep 13;60(38):20833-20839. doi:, 10.1002/anie.202106256. Epub 2021 Aug 17. PMID:34288320^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.