7jm7

From Proteopedia

Jump to: navigation, search

Structure of human CLC-7/OSTM1 complex

Structural highlights

7jm7 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 2.82Å
Ligands:0J1, ATP, BMA, CL, MAN, MG, NAG
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

OSTM1_HUMAN Infantile osteopetrosis with neuroaxonal dysplasia. The disease is caused by variants affecting the gene represented in this entry.

Function

OSTM1_HUMAN Required for osteoclast and melanocyte maturation and function.[1]

Publication Abstract from PubMed

The chloride-proton exchanger CLC-7 plays critical roles in lysosomal homeostasis and bone regeneration and its mutation can lead to osteopetrosis, lysosomal storage disease and neurological disorders. In lysosomes and the ruffled border of osteoclasts, CLC-7 requires a beta-subunit, OSTM1, for stability and activity. Here, we present electron cryomicroscopy structures of CLC-7 in occluded states by itself and in complex with OSTM1, determined at resolutions up to 2.8 A. In the complex, the luminal surface of CLC-7 is entirely covered by a dimer of the heavily glycosylated and disulfide-bonded OSTM1, which serves to protect CLC-7 from the degradative environment of the lysosomal lumen. OSTM1 binding does not induce large-scale rearrangements of CLC-7, but does have minor effects on the conformation of the ion-conduction pathway, potentially contributing to its regulatory role. These studies provide insights into the role of OSTM1 and serve as a foundation for understanding the mechanisms of CLC-7 regulation.

Cryo-EM structure of the lysosomal chloride-proton exchanger CLC-7 in complex with OSTM1.,Schrecker M, Korobenko J, Hite RK Elife. 2020 Aug 4;9. pii: 59555. doi: 10.7554/eLife.59555. PMID:32749217[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Loading citation details..
Citations
No citations found

See Also

References

  1. Leisle L, Ludwig CF, Wagner FA, Jentsch TJ, Stauber T. ClC-7 is a slowly voltage-gated 2Cl(-)/1H(+)-exchanger and requires Ostm1 for transport activity. EMBO J. 2011 Jun 1;30(11):2140-52. PMID:21527911 doi:10.1038/emboj.2011.137
  2. Schrecker M, Korobenko J, Hite RK. Cryo-EM structure of the lysosomal chloride-proton exchanger CLC-7 in complex with OSTM1. Elife. 2020 Aug 4;9:e59555. PMID:32749217 doi:10.7554/eLife.59555

Contents


PDB ID 7jm7

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools