7l36

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PEPCK steady-state structure with Mn and GTP

Structural highlights

7l36 is a 1 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.84Å
Ligands:CL, GTP, MN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PCKGC_RAT Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Publication Abstract from PubMed

Time-resolved crystallography of biomolecules in action has advanced rapidly as methods for serial crystallography have improved, but the large number of crystals and the complex experimental infrastructure that are required remain serious obstacles to its widespread application. Here, millisecond mix-and-quench crystallography (MMQX) has been developed, which yields millisecond time-resolved data using far fewer crystals and routine remote synchrotron data collection. To demonstrate the capabilities of MMQX, the conversion of oxaloacetic acid to phosphoenolpyruvate by phosphoenolpyruvate carboxy-kinase (PEPCK) is observed with a time resolution of 40 ms. By lowering the entry barrier to time-resolved crystallography, MMQX should enable a broad expansion in structural studies of protein dynamics.

Millisecond mix-and-quench crystallography (MMQX) enables time-resolved studies of PEPCK with remote data collection.,Clinger JA, Moreau DW, McLeod MJ, Holyoak T, Thorne RE IUCrJ. 2021 Aug 4;8(Pt 5):784-792. doi: 10.1107/S2052252521007053. eCollection, 2021 Sep 1. PMID:34584739[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
1 reviews cite this structure
Mapping Enzyme Landscapes by Time-Resolved Crystallography with Synchrotron and X-Ray Free Electron Laser Light.
Wilson et al. (2022)
0 more
2 other articles
No citations found

See Also

References

  1. ↑ Clinger JA, Moreau DW, McLeod MJ, Holyoak T, Thorne RE. Millisecond mix-and-quench crystallography (MMQX) enables time-resolved studies of PEPCK with remote data collection. IUCrJ. 2021 Aug 4;8(Pt 5):784-792. PMID:34584739 doi:10.1107/S2052252521007053

Contents


PDB ID 7l36

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