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7m6m

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Full length alpha1 Glycine receptor in presence of 32uM Tetrahydrocannabinol

Structural highlights

7m6m is a 5 chain structure with sequence from Danio rerio. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.09Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLRA1_DANRE Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:10188956, PubMed:26344198). Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol (By similarity).[UniProtKB:P23415]^[1] ^[2]

Publication Abstract from PubMed

Nociception and motor coordination are critically governed by glycine receptor (GlyR) function at inhibitory synapses. Consequentially, GlyRs are attractive targets in the management of chronic pain and in the treatment of several neurological disorders. High-resolution mechanistic details of GlyR function and its modulation are just emerging. While it has been known that cannabinoids such as Delta(9)-tetrahydrocannabinol (THC), the principal psychoactive constituent in marijuana, potentiate GlyR in the therapeutically relevant concentration range, the molecular mechanism underlying this effect is still not understood. Here, we present Cryo-EM structures of full-length GlyR reconstituted into lipid nanodisc in complex with THC under varying concentrations of glycine. The GlyR-THC complexes are captured in multiple conformational states that reveal the basis for THC-mediated potentiation, manifested as different extents of opening at the level of the channel pore. Taken together, these structural findings, combined with molecular dynamics simulations and functional analysis, provide insights into the potential THC binding site and the allosteric coupling to the channel pore.

Structural basis for cannabinoid-induced potentiation of alpha1-glycine receptors in lipid nanodiscs.,Kumar A, Kindig K, Rao S, Zaki AM, Basak S, Sansom MSP, Biggin PC, Chakrapani S Nat Commun. 2022 Aug 18;13(1):4862. doi: 10.1038/s41467-022-32594-5. PMID:35982060^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ David-Watine B, Goblet C, de Saint Jan D, Fucile S, Devignot V, Bregestovski P, Korn H. Cloning, expression and electrophysiological characterization of glycine receptor alpha subunit from zebrafish. Neuroscience. 1999 Apr;90(1):303-17. doi: 10.1016/s0306-4522(98)00430-8. PMID:10188956 doi:http://dx.doi.org/10.1016/s0306-4522(98)00430-8
↑ Du J, Lu W, Wu S, Cheng Y, Gouaux E. Glycine receptor mechanism elucidated by electron cryo-microscopy. Nature. 2015 Sep 7. doi: 10.1038/nature14853. PMID:26344198 doi:http://dx.doi.org/10.1038/nature14853
↑ Kumar A, Kindig K, Rao S, Zaki AM, Basak S, Sansom MSP, Biggin PC, Chakrapani S. Structural basis for cannabinoid-induced potentiation of alpha1-glycine receptors in lipid nanodiscs. Nat Commun. 2022 Aug 18;13(1):4862. doi: 10.1038/s41467-022-32594-5. PMID:35982060 doi:http://dx.doi.org/10.1038/s41467-022-32594-5