| Structural highlights
Function
EF2K_HUMAN Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.[1] [2]
Publication Abstract from PubMed
Translation is a tightly regulated process that ensures optimal protein quality and enables adaptation to energy/nutrient availability. The alpha-kinase eukaryotic elongation factor 2 kinase (eEF-2K), a key regulator of translation, specifically phosphorylates the guanosine triphosphatase eEF-2, thereby reducing its affinity for the ribosome and suppressing the elongation phase of protein synthesis. eEF-2K activation requires calmodulin binding and autophosphorylation at the primary stimulatory site, T348. Biochemical studies predict a calmodulin-mediated activation mechanism for eEF-2K distinct from other calmodulin-dependent kinases. Here, we resolve the atomic details of this mechanism through a 2.3-A crystal structure of the heterodimeric complex of calmodulin and the functional core of eEF-2K (eEF-2K(TR)). This structure, which represents the activated T348-phosphorylated state of eEF-2K(TR), highlights an intimate association of the kinase with the calmodulin C-lobe, creating an "activation spine" that connects its amino-terminal calmodulin-targeting motif to its active site through a conserved regulatory element.
Structural basis for the calmodulin-mediated activation of eukaryotic elongation factor 2 kinase.,Piserchio A, Isiorho EA, Long K, Bohanon AL, Kumar EA, Will N, Jeruzalmi D, Dalby KN, Ghose R Sci Adv. 2022 Jul 8;8(27):eabo2039. doi: 10.1126/sciadv.abo2039. Epub 2022 Jul 6. PMID:35857468[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Browne GJ, Finn SG, Proud CG. Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398. J Biol Chem. 2004 Mar 26;279(13):12220-31. Epub 2004 Jan 5. PMID:14709557 doi:10.1074/jbc.M309773200
- ↑ Ryazanov AG, Ward MD, Mendola CE, Pavur KS, Dorovkov MV, Wiedmann M, Erdjument-Bromage H, Tempst P, Parmer TG, Prostko CR, Germino FJ, Hait WN. Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. Proc Natl Acad Sci U S A. 1997 May 13;94(10):4884-9. PMID:9144159
- ↑ Piserchio A, Isiorho EA, Long K, Bohanon AL, Kumar EA, Will N, Jeruzalmi D, Dalby KN, Ghose R. Structural basis for the calmodulin-mediated activation of eukaryotic elongation factor 2 kinase. Sci Adv. 2022 Jul 8;8(27):eabo2039. doi: 10.1126/sciadv.abo2039. Epub 2022 Jul 6. PMID:35857468 doi:http://dx.doi.org/10.1126/sciadv.abo2039
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