Structural highlights
Function
PRI2_HUMAN DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.
Publication Abstract from PubMed
The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase alpha subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 A human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA-DNA primers.
Structures of human primosome elongation complexes.,He Q, Baranovskiy AG, Morstadt LM, Lisova AE, Babayeva ND, Lusk BL, Lim CJ, Tahirov TH Nat Struct Mol Biol. 2023 May;30(5):579-583. doi: 10.1038/s41594-023-00971-3. , Epub 2023 Apr 17. PMID:37069376[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ He Q, Baranovskiy AG, Morstadt LM, Lisova AE, Babayeva ND, Lusk BL, Lim CJ, Tahirov TH. Structures of human primosome elongation complexes. Nat Struct Mol Biol. 2023 May;30(5):579-583. PMID:37069376 doi:10.1038/s41594-023-00971-3
