8efy

From Proteopedia

Jump to: navigation, search

Structure of double homo-hexameric AAA+ ATPase RuvB motors

Structural highlights

8efy is a 16 chain structure with sequence from Thermus thermophilus HB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.16Å
Ligands:ADP, AGS, MG
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RUVB_THET8 The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg(2+)-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone.[1]

Publication Abstract from PubMed

The Holliday junction (HJ) is a DNA intermediate of homologous recombination, involved in many fundamental physiological processes. RuvB, an ATPase motor protein, drives branch migration of the Holliday junction with a mechanism that had yet to be elucidated. Here we report two cryo-EM structures of RuvB, providing a comprehensive understanding of HJ branch migration. RuvB assembles into a spiral staircase, ring-like hexamer, encircling dsDNA. Four protomers of RuvB contact the DNA backbone with a translocation step size of 2 nucleotides. The variation of nucleotide-binding states in RuvB supports a sequential model for ATP hydrolysis and nucleotide recycling, which occur at separate, singular positions. RuvB's asymmetric assembly also explains the 6:4 stoichiometry between the RuvB/RuvA complex, which coordinates HJ migration in bacteria. Taken together, we provide a mechanistic understanding of HJ branch migration facilitated by RuvB, which may be universally shared by prokaryotic and eukaryotic organisms.

Molecular mechanisms of Holliday junction branch migration catalyzed by an asymmetric RuvB hexamer.,Rish AD, Shen Z, Chen Z, Zhang N, Zheng Q, Fu TM Nat Commun. 2023 Jun 15;14(1):3549. doi: 10.1038/s41467-023-39250-6. PMID:37322069[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Loading citation details..
Citations
0 reviews cite this structure
Molecular and structural basis of an ATPase-nuclease dual-enzyme anti-phage defense complex.
An et al. (2024)
-1 more
0 other articles
No citations found

References

  1. ↑ Yamada K, Fukuoh A, Iwasaki H, Shinagawa H. Novel properties of the Thermus thermophilus RuvB protein, which promotes branch migration of Holliday junctions. Mol Gen Genet. 1999 Jul;261(6):1001-11. PMID:10485292
  2. ↑ Rish AD, Shen Z, Chen Z, Zhang N, Zheng Q, Fu TM. Molecular mechanisms of Holliday junction branch migration catalyzed by an asymmetric RuvB hexamer. Nat Commun. 2023 Jun 15;14(1):3549. PMID:37322069 doi:10.1038/s41467-023-39250-6

Contents


PDB ID 8efy

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/8efy"
Personal tools