8h0n

Structural highlights

Disease

WDR4_HUMAN Galloway-Mowat syndrome. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry.

Function

WDR4_HUMAN Non-catalytic component of a methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:12403464, PubMed:31031084, PubMed:31031083). In the methyltransferase complex, it is required to stabilize and induce conformational changes of the catalytic subunit (PubMed:12403464). Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (PubMed:12403464, PubMed:31031084). Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs (PubMed:31031084). Also required for methylation of a specific subset of miRNAs, such as let-7 (PubMed:31031083). Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed:26751069).^{[1] [2] [3] [4]}

References

1. ↑ Alexandrov A, Martzen MR, Phizicky EM. Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA. RNA. 2002 Oct;8(10):1253-66. PMID:12403464
2. ↑ Cheng IC, Chen BC, Shuai HH, Chien FC, Chen P, Hsieh TS. Wuho Is a New Member in Maintaining Genome Stability through its Interaction with Flap Endonuclease 1. PLoS Biol. 2016 Jan 11;14(1):e1002349. doi: 10.1371/journal.pbio.1002349. , eCollection 2016 Jan. PMID:26751069 doi:http://dx.doi.org/10.1371/journal.pbio.1002349
3. ↑ Pandolfini L, Barbieri I, Bannister AJ, Hendrick A, Andrews B, Webster N, Murat P, Mach P, Brandi R, Robson SC, Migliori V, Alendar A, d'Onofrio M, Balasubramanian S, Kouzarides T. METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation. Mol Cell. 2019 Jun 20;74(6):1278-1290.e9. doi: 10.1016/j.molcel.2019.03.040. Epub , 2019 Apr 25. PMID:31031083 doi:http://dx.doi.org/10.1016/j.molcel.2019.03.040
4. ↑ Zhang LS, Liu C, Ma H, Dai Q, Sun HL, Luo G, Zhang Z, Zhang L, Hu L, Dong X, He C. Transcriptome-wide Mapping of Internal N(7)-Methylguanosine Methylome in Mammalian mRNA. Mol Cell. 2019 Jun 20;74(6):1304-1316.e8. doi: 10.1016/j.molcel.2019.03.036. Epub , 2019 Apr 25. PMID:31031084 doi:http://dx.doi.org/10.1016/j.molcel.2019.03.036