We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

8iex

From Proteopedia

Jump to: navigation, search

Solution structure of AtWRKY11-DBD

Structural highlights

8iex is a 1 chain structure with sequence from Arabidopsis thaliana. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 20 models
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

WRK11_ARATH Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000, probably by activating the jasmonic acid (JA)- signaling pathway (PubMed:26433201).^[1]

Publication Abstract from PubMed

The Arabidopsis WRKY11 (AtWRKY11) protein is an important transcription factor involved in plant response to biotic and abiotic stresses. Its DNA-binding domain specifically binds to gene promoter regions harboring the W-box consensus motif. Herein we report the high-resolution structure of the AtWRKY11 DNA-binding domain (DBD) determined by solution NMR spectroscopy. The results show that AtWRKY11-DBD adopts an all-beta fold comprising five beta-strands packed in an antiparallel topology, stabilized by a zinc-finger motif. Structural comparison reveals that the long beta(1)-beta(2) loop shows the highest structural variation from other available WRKY domain structures. Moreover, this loop was further found to contribute to the binding between AtWRKY11-DBD and W-box DNA. Our current study provides atomic-level structural basis for further understanding the structure-function relationship of plant WRKY proteins.

Solution structure of the DNA binding domain of Arabidopsis transcription factor WRKY11.,Wang J, Lin Y, Yang J, Zhang Q, Liu M, Hu Y, Dong X Biochem Biophys Res Commun. 2023 Apr 23;653:133-139. doi: , 10.1016/j.bbrc.2023.02.072. Epub 2023 Feb 26. PMID:36868077^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jiang CH, Huang ZY, Xie P, Gu C, Li K, Wang DC, Yu YY, Fan ZH, Wang CJ, Wang YP, Guo YH, Guo JH. Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis. J Exp Bot. 2016 Jan;67(1):157-74. PMID:26433201 doi:10.1093/jxb/erv445
↑ Wang J, Lin Y, Yang J, Zhang Q, Liu M, Hu Y, Dong X. Solution structure of the DNA binding domain of Arabidopsis transcription factor WRKY11. Biochem Biophys Res Commun. 2023 Apr 23;653:133-139. PMID:36868077 doi:10.1016/j.bbrc.2023.02.072