8oiv
From Proteopedia
Monkeypox virus VP39 in complex with SAH and cap0
Structural highlights
FunctionA0A0F6N8X1_MONPV Displays methyltransferase, positive regulation of the poly(A) polymerase and transcription elongation activities. Involved in the modification of both mRNA ends and in intermediate and late gene positive transcription elongation. At the mRNAs 5' end, methylates the ribose 2' OH group of the first transcribed nucleotide, thereby producing a 2'-O-methylpurine cap. At the 3' end, functions as a processivity factor which stimulates the activity of the viral poly(A) polymerase VP55 that creates mRNA's poly(A) tail. In the presence of VP39, VP55 does not dissociate from the RNA allowing tail elongation to around 250 adenylates.[ARBA:ARBA00003680][PIRNR:PIRNR003726] Publication Abstract from PubMedMpox is a zoonotic disease caused by the mpox virus (MPXV), which has gained attention due to its rapid and widespread transmission, with reports from more than 100 countries. The virus belongs to the Orthopoxvirus genus, which also includes variola virus and vaccinia virus. In poxviruses, the RNA cap is crucial for the translation and stability of viral mRNAs and also for immune evasion. This study presents the crystal structure of the mpox 2'-O-methyltransfarase VP39 in complex with a short cap-0 RNA. The RNA substrate binds to the protein without causing any significant changes to its overall fold and is held in place by a combination of electrostatic interactions, pi-pi stacking and hydrogen bonding. The structure also explains the mpox VP39 preference for a guanine base at the first position; it reveals that guanine forms a hydrogen bond that an adenine would not be able to form. Structural basis for RNA-cap recognition and methylation by the mpox methyltransferase VP39.,Skvara P, Chalupska D, Klima M, Kozic J, Silhan J, Boura E Antiviral Res. 2023 Aug;216:105663. doi: 10.1016/j.antiviral.2023.105663. Epub , 2023 Jul 7. PMID:37421984[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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