8x4n

Structural highlights

Function

SETA_LEGPH Secreted effector that interferes with vesicular trafficking of host cells. Possesses glucohydrolase and mono-O-glucosyltransferase activity by using UDP-glucose as a sugar donor substrate. Is able to glucosylate histones H4 and H3.1 in vitro, but it is unlikely that histones are the natural substrates for SetA. May glycosylate a component of the host cell vesicle trafficking machinery during L.pneumophila infection. Binds with high specificity to phosphatidylinositol 3-phosphate (PtdIns(3)P), (with a dissociation constant value of 809 nM), which guides SetA to the cytosolic leaflet of the early phagosome of the host cell.^{[1] [2]}

References

1. ↑ Heidtman M, Chen EJ, Moy MY, Isberg RR. Large-scale identification of Legionella pneumophila Dot/Icm substrates that modulate host cell vesicle trafficking pathways. Cell Microbiol. 2009 Feb;11(2):230-48. PMID:19016775 doi:10.1111/j.1462-5822.2008.01249.x
2. ↑ Jank T, Böhmer KE, Tzivelekidis T, Schwan C, Belyi Y, Aktories K. Domain organization of Legionella effector SetA. Cell Microbiol. 2012 Jun;14(6):852-68. PMID:22288428 doi:10.1111/j.1462-5822.2012.01761.x