Structural highlights
Function
CLH_YEAST Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. In yeast, it is involved in the retention of proteins in an intracellular membrane compartment, presumably the trans-Golgi.[1]
Publication Abstract from PubMed
Clathrin forms a triskelion, or three-legged, network that regulates cellular processes by facilitating cargo internalization and trafficking in eukaryotes. Its N-terminal domain is crucial for interacting with adaptor proteins, which link clathrin to the membrane and engage with specific cargo. The N-terminal domain contains up to four adaptor-binding sites, though their role in preferential occupancy by adaptor proteins remains unclear. In this study, we examine the binding hierarchy of adaptors for clathrin, using integrative biophysical and structural approaches, along with in vivo functional experiments. We find that yeast epsin Ent5 has the highest affinity for clathrin, highlighting its key role in cellular trafficking. Epsins Ent1 and Ent2, crucial for endocytosis but thought to have redundant functions, show distinct binding patterns. Ent1 exhibits stronger interactions with clathrin than Ent2, suggesting a functional divergence toward actin binding. These results offer molecular insights into adaptor protein selectivity, suggesting they competitively bind clathrin while also targeting three different clathrin sites.
Subtleties in Clathrin heavy chain binding boxes provide selectivity among adaptor proteins of budding yeast.,Defelipe LA, Veith K, Burastero O, Kupriianova T, Bento I, Skruzny M, Kolbel K, Uetrecht C, Thuenauer R, Garcia-Alai MM Nat Commun. 2024 Nov 7;15(1):9655. doi: 10.1038/s41467-024-54037-z. PMID:39511183[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ha SA, Torabinejad J, DeWald DB, Wenk MR, Lucast L, De Camilli P, Newitt RA, Aebersold R, Nothwehr SF. The synaptojanin-like protein Inp53/Sjl3 functions with clathrin in a yeast TGN-to-endosome pathway distinct from the GGA protein-dependent pathway. Mol Biol Cell. 2003 Apr;14(4):1319-33. PMID:12686590 doi:10.1091/mbc.e02-10-0686
- ↑ Defelipe LA, Veith K, Burastero O, Kupriianova T, Bento I, Skruzny M, Kölbel K, Uetrecht C, Thuenauer R, García-Alai MM. Subtleties in Clathrin heavy chain binding boxes provide selectivity among adaptor proteins of budding yeast. Nat Commun. 2024 Nov 7;15(1):9655. PMID:39511183 doi:10.1038/s41467-024-54037-z
