Dead-box RNA helicase DDX19, in complex with an ATP-analogue and RNA

Human dead-box RNA helicase DDX19, in complex with an ATP-analogue and RNA (3g0h)

About this Structure

3G0H is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

bound. .

DDX19 is .

• With RNA and the ATP analog bound, the N-terminal extension (dark green) is tucked at the side and the two domains are close together.
• This conformation is presumed to resemble the pre-hydrolysis state in which RNA is bound. It is also referred to as the closed state and closed cleft conformation.
• This scene is similar to the view in Figure 1C of the paper describing the structure.

Showing is bound.

• This overlay clearly illustrates the dramatic shift in the location of the N-terminal extension (dark green).
• This view clearly shows how the N-terminal helix of the extension extends from the RNA-binding site to down near the ATP-binding site.

. The placement of the N-terminal extension between the two helicase domains negatively regulates ATPase activity. A .

• Of particular note is arginine 429, the so-called arginine finger, that is essential for ATPase activity. for ATPase function.
• illustrates how it is moved away from the active site when RNA is not bound and hydrolysis has occurred to produce ADP.
• Keep in mind the intermediate models, in between the endpoints, are hypothetical.

See also

• 3ews is human DDX19 with ADP bound. It is used in the morphs here and was described in the same article as 3g0h.
• Helicase