Ectonucleoside triphosphate diphosphohydrolase

From Proteopedia

spinqualitylabelsall models Structure of NTDPase1 complex with GMPPNP, Na+ (purple), Cl- (large green), Mg+2 (small green) ions (PDB code 4brg). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Ectonucleoside triphosphate diphosphohydrolase (NTPDase) hydrolyzes nucleoside 5’-triphosphate to nucleoside 5’-phosphate and 2 phosphates. NTPDase can hydrolyze ATP and ADP. NTPDase has an important role in regulating neutrophil chemotaxis. NTPDase 1,2,3,8 are cell surface enzymes with catalytic sites facing extracellularly while other NTPDases act intracellularly. NTPDase require Ca+2 or Mg+2 for their activity. The NTPDases can be differentiated according to their substrate specificity, divalent cation usage and product formation.[1] NTPDase 1 hydrolyzes ATP released by neutrophils[2]. NTPDase 2 hydrolyzes extracellular ATP [3]. NTPDase 3 hydrolyzes ATP in pancreatic beta-cells[4]. NTPDase 4 hydrolyzes ATP in Golgi bodies[5].

3D structures of ectonucleoside triphosphate diphosphohydrolase

Updated on 16-June-2024

References

1. ↑ Zebisch M, Krauss M, Schafer P, Lauble P, Strater N. Crystallographic Snapshots along the Reaction Pathway of Nucleoside Triphosphate Diphosphohydrolases. Structure. 2013 Jul 2. pii: S0969-2126(13)00200-1. doi:, 10.1016/j.str.2013.05.016. PMID:23830739 doi:10.1016/j.str.2013.05.016
2. ↑ Corriden R, Chen Y, Inoue Y, Beldi G, Robson SC, Insel PA, Junger WG. Ecto-nucleoside triphosphate diphosphohydrolase 1 (E-NTPDase1/CD39) regulates neutrophil chemotaxis by hydrolyzing released ATP to adenosine. J Biol Chem. 2008 Oct 17;283(42):28480-6. PMID:18713747 doi:10.1074/jbc.M800039200
3. ↑ Dragic M, Mihajlovic K, Adzic M, Jakovljevic M, Kontic MZ, Mitrović N, Laketa D, Lavrnja I, Kipp M, Grković I, Nedeljkovic N. Expression of Ectonucleoside Triphosphate Diphosphohydrolase 2 (NTPDase2) Is Negatively Regulated Under Neuroinflammatory Conditions In Vivo and In Vitro. ASN Neuro. 2022 Jan-Dec;14:17590914221102068. PMID:35593054 doi:10.1177/17590914221102068
4. ↑ Syed SK, Kauffman AL, Beavers LS, Alston JT, Farb TB, Ficorilli J, Marcelo MC, Brenner MB, Bokvist K, Barrett DG, Efanov AM. Ectonucleotidase NTPDase3 is abundant in pancreatic β-cells and regulates glucose-induced insulin secretion. Am J Physiol Endocrinol Metab. 2013 Nov 15;305(10):E1319-26. PMID:24085034 doi:10.1152/ajpendo.00328.2013
5. ↑ Gorelik A, Labriola JM, Illes K, Nagar B. Crystal structure of the nucleotide-metabolizing enzyme NTPDase4. Protein Sci. 2020 Aug 6. doi: 10.1002/pro.3926. PMID:32767432 doi:http://dx.doi.org/10.1002/pro.3926