T7 RNA Polymerase

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T7 RNA Polymerase Initiation Complex

Promoter bound polymerase (PDB code 1qln)

Files for 3D printer

T7 RNA polymerase model by Marius Mihasan

References

1,"1aro","Free enz plus lysozyme","T7RP with the bound inhibitor T7 lysozyme, no DNA - Jeruzalmi, D. & Steitz, T. A. (1998) EMBO J 17, 4101-4113"

2,"1cez","Enz with DNA bound (ED complex)","Early structure of T7RP with promo bound - Cheetham, G. M., Jeruzalmi, D. & Steitz, T. A. (1999) Nature 399, 80-83"

3,"2pi5","Enz with DNA bound (ED complex)","T7RP with promoter and first two NTPs bound - Kennedy, W.P.,††Momand, J.R.,††Yin, Y.W. (2007) Mechanism for de novo RNA synthesis and initiating nucleotide specificity by t7 RNA polymerase. J.Mol.Biol. 370: 256-268"

4,"2pi4","ED complex with GTP + GTP","T7RP with promoter and first two NTPs bound - Kennedy, W.P.,††Momand, J.R.,††Yin, Y.W. (2007) Mechanism for de novo RNA synthesis and initiating nucleotide specificity by t7 RNA polymerase. J.Mol.Biol. 370: 256-268"

5,"1qln","ED with 3mer RNA","T7RP with promoter DNA and GTP, allowing formation of a 3 base transcript - Cheetham, G. M. & Steitz, T. A. (1999) Science 286, 2305-2309",true

7,"3e2e","Initial complex at +7","The structure of a transcribing T7 RNA polymerase in transition from initiation to elongation - Durniak, K.J., Bailey, S., Steitz, T.A. (2008) Science 322, 553-7"

6,"3e3j","Initial complex at +8","The structure of a transcribing T7 RNA polymerase in transition from initiation to elongation - Durniak, K.J., Bailey, S., Steitz, T.A. (2008) Science 322, 553-7"

8,"1msw","Elongation complex (Steitz)","Elongation complex model formed with mismatch bubble DNA - Yin, Y. W. & Steitz, T. A. (2002). Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase. Science 298, 1387-1395."

9,"1h38","Elongation w scaffold","Elongation complex model formed by multi-piece scaffold - Tahirov, T. H., Temiakov, D., Anikin, M., Patlan, V., McAllister, W. T., Vassylyev, D. G. & Yokoyama, S. (2002) Nature 420, 43-50"

10,"1s0v","Elongation w ab-me-ATP","Scaffold elongation complex with non-hydrolyzable substrate NTP - Temiakov, D., Patlan, V., Anikin, M., McAllister, W. T., Yokoyama, S. & Vassylyev, D. G. (2004) Cell 116, 381-391"

11,"1s76","Elongation w ab-me-ATP","Mismatched bubble elongation complex with non-hydrolyzable substrate NTP - Yin, Y. W. & Steitz, T. A. (2004) Cell 116, 393-404"

12,"1s77","Elongation w PPi","Yin, Y. W. & Steitz, T. A. (2004) Cell 116, 393-404"

13,"4rnp","Low res free enz"

  1. The Storymorph Jmol scripts were used to create the interpolation shown in the morph. Coordinates available on Proteopedia

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