1qmw
From Proteopedia
(New page: 200px<br /><applet load="1qmw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qmw" /> '''SOLUTION STRUCTURE OF ALPHA-CONOTOXIN SI'''<...) |
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| - | [[Image:1qmw.jpg|left|200px]]<br /><applet load="1qmw" size=" | + | [[Image:1qmw.jpg|left|200px]]<br /><applet load="1qmw" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''SOLUTION STRUCTURE OF ALPHA-CONOTOXIN SI'''<br /> | '''SOLUTION STRUCTURE OF ALPHA-CONOTOXIN SI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The nuclear magnetic resonance solution structure of alpha-conotoxin SI | + | The nuclear magnetic resonance solution structure of alpha-conotoxin SI has been determined at pH 4.2. The 36 lowest energy structures show that alpha-conotoxin SI exists in a single major solution conformation and is stabilized by six hydrogen bonds. Comparisons are made between the SI solution structure and the solution and crystal structures of alpha-conotoxin GI. Surprisingly, a high degree of similarity between the backbone conformations of the GI crystal and the SI solution structures is seen in the region of lowest sequence homology, namely residues Gly-8 to Ser-12. This similarity is more surprising when considering that in SI a proline replaces the Arg-9 found in GI. The correspondence in conformation in this region provides the definitive evidence that it is the loss of the arginine basic charge at residue 9 which determines the differences in toxicity between GI and SI, rather than any changes in conformation induced by the cyclic proline residue. |
==About this Structure== | ==About this Structure== | ||
| - | 1QMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_striatus Conus striatus] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1QMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_striatus Conus striatus] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QMW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Barany, G.]] | [[Category: Barany, G.]] | ||
| - | [[Category: Benie, A | + | [[Category: Benie, A J.]] |
[[Category: Hargittai, B.]] | [[Category: Hargittai, B.]] | ||
| - | [[Category: Janes, R | + | [[Category: Janes, R W.]] |
[[Category: Whitford, D.]] | [[Category: Whitford, D.]] | ||
[[Category: NH2]] | [[Category: NH2]] | ||
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[[Category: venom]] | [[Category: venom]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:41:29 2008'' |
Revision as of 12:41, 21 February 2008
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SOLUTION STRUCTURE OF ALPHA-CONOTOXIN SI
Overview
The nuclear magnetic resonance solution structure of alpha-conotoxin SI has been determined at pH 4.2. The 36 lowest energy structures show that alpha-conotoxin SI exists in a single major solution conformation and is stabilized by six hydrogen bonds. Comparisons are made between the SI solution structure and the solution and crystal structures of alpha-conotoxin GI. Surprisingly, a high degree of similarity between the backbone conformations of the GI crystal and the SI solution structures is seen in the region of lowest sequence homology, namely residues Gly-8 to Ser-12. This similarity is more surprising when considering that in SI a proline replaces the Arg-9 found in GI. The correspondence in conformation in this region provides the definitive evidence that it is the loss of the arginine basic charge at residue 9 which determines the differences in toxicity between GI and SI, rather than any changes in conformation induced by the cyclic proline residue.
About this Structure
1QMW is a Single protein structure of sequence from Conus striatus with as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of alpha-conotoxin SI., Benie AJ, Whitford D, Hargittai B, Barany G, Janes RW, FEBS Lett. 2000 Jul 7;476(3):287-95. PMID:10913630
Page seeded by OCA on Thu Feb 21 14:41:29 2008
