1vif
From Proteopedia
(New page: 200px<br /><applet load="1vif" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vif, resolution 1.8Å" /> '''STRUCTURE OF DIHYDROF...) |
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| - | [[Image:1vif.jpg|left|200px]]<br /><applet load="1vif" size=" | + | [[Image:1vif.jpg|left|200px]]<br /><applet load="1vif" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vif, resolution 1.8Å" /> | caption="1vif, resolution 1.8Å" /> | ||
'''STRUCTURE OF DIHYDROFOLATE REDUCTASE'''<br /> | '''STRUCTURE OF DIHYDROFOLATE REDUCTASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Bacteria expressing R67-plasmid encoded dihydrofolate reductase (R67 DHFR) | + | Bacteria expressing R67-plasmid encoded dihydrofolate reductase (R67 DHFR) exhibit high-level resistance to the antibiotic trimethoprim. Native R67 DHFR is a 34,000 M(r) homotetramer which exists in equilibrium with an inactive dimeric form. The structure of native R67 DHFR has now been solved at 1.7 A resolution and is unrelated to that of chromosomal DHFR. Homotetrameric R67 DHFR has an unusual pore, 25 A in length, passing through the middle of the molecule. Two folate molecules bind asymmetrically within the pore indicating that the enzyme's active site consists of symmetry related binding surfaces from all four identical units. |
==About this Structure== | ==About this Structure== | ||
| - | 1VIF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with FOL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] Full crystallographic information is available from [http:// | + | 1VIF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=FOL:'>FOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VIF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Howell, E | + | [[Category: Howell, E E.]] |
| - | [[Category: Matthews, D | + | [[Category: Matthews, D A.]] |
[[Category: Narayana, N.]] | [[Category: Narayana, N.]] | ||
| - | [[Category: Xuong, N | + | [[Category: Xuong, N H.]] |
[[Category: FOL]] | [[Category: FOL]] | ||
[[Category: nadp]] | [[Category: nadp]] | ||
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[[Category: trimethoprim resistance methotrexate resistance]] | [[Category: trimethoprim resistance methotrexate resistance]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:35:38 2008'' |
Revision as of 13:35, 21 February 2008
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STRUCTURE OF DIHYDROFOLATE REDUCTASE
Overview
Bacteria expressing R67-plasmid encoded dihydrofolate reductase (R67 DHFR) exhibit high-level resistance to the antibiotic trimethoprim. Native R67 DHFR is a 34,000 M(r) homotetramer which exists in equilibrium with an inactive dimeric form. The structure of native R67 DHFR has now been solved at 1.7 A resolution and is unrelated to that of chromosomal DHFR. Homotetrameric R67 DHFR has an unusual pore, 25 A in length, passing through the middle of the molecule. Two folate molecules bind asymmetrically within the pore indicating that the enzyme's active site consists of symmetry related binding surfaces from all four identical units.
About this Structure
1VIF is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Dihydrofolate reductase, with EC number 1.5.1.3 Full crystallographic information is available from OCA.
Reference
A plasmid-encoded dihydrofolate reductase from trimethoprim-resistant bacteria has a novel D2-symmetric active site., Narayana N, Matthews DA, Howell EE, Nguyen-huu X, Nat Struct Biol. 1995 Nov;2(11):1018-25. PMID:7583655
Page seeded by OCA on Thu Feb 21 15:35:38 2008
